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| Status |
Public on Mar 01, 2020 |
| Title |
Sequencing of commercially-available pancreatic cancer cell lines reveals selective CYP3A5 overexpression among xenobiotic-metabolizing CYPs. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: To know the expression of all cytochrome P450 (CYP) enzymes and related drug metabolizing enzymes in pancreatic cancer models
Methods: We obtained expression profiles of 8 commercially-available pancreatic cancer cell lines and one non-cancerous immortalized cell line by RNA-sequencing. The pancreatic cancer cells were compared among themselves and to the non-cancerous control cells.
Results: We confirmed reports that CYP3A5 is highly overexpressed in pancreatic cancer models, and additionally report that known xenobiotic-metabolizing CYPs are lowly expressed in comparison.
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| Overall design |
mRNA profiles of each cell line were obtaiend in triplicate using Illumina-based RNA-Sequencing. Sequencing was conducted as paired-end 100bp reads. The HPNE (non-cancerous pancreatic cells) group is considered as the control.
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| Contributor(s) |
Wright WC, Oladimeji PO, Chen T |
| Citation(s) |
31965799 |
| Submission date |
Oct 04, 2019 |
| Last update date |
Nov 22, 2021 |
| Contact name |
William C. Wright |
| E-mail(s) |
charlie.wright@stjude.org
|
| Organization name |
St. Jude Children's Research Hospital
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| Department |
Computational Biology
|
| Street address |
262 Danny Thomas Pl
|
| City |
Memphis |
| State/province |
Tennessee |
| ZIP/Postal code |
38105 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA575858 |
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