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Series GSE138378 Query DataSets for GSE138378
Status Public on Dec 01, 2019
Title The histone modification landscape of neonatal CD4+ T cells contributes to decreased T cell receptor signaling
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Chromatin immunoprecipitation with massively parallel DNA sequencing (ChIP-seq) was performed to establish the H3K4me3 landscape in neonatal and adult naive CD4+ T cells. As development progressed from neonate to adult, naive CD4+ T cells gained the activating mark H3K4me3 at immunologically important promoter sites. Adult naive CD4+ T cells had enrichment of H3K4me3 at genes important in T cell receptor signaling, which was associated with improved markers of activation and cytokine expression in adult naive CD4+ T cells after engagement of the T cell receptor.
 
Overall design Examination of H3K4me3 in naïve CD4+ T cells in 7 experimental groups, each in triplicate: under 30 weeks gestation preterm healthy neonate, under 30 weeks gestation preterm chorioamnionitis-exposed neonate, 30-36 weeks gestation preterm healthy neonate, 30-36 weeks gestation preterm chorioamnionitis-exposed neonate, over 37 weeks gestation term healthy neonate, over 37 weeks gestation term chorioamnionitis-exposed neonate, healthy adult
 
Contributor(s) Bermick J
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Submission date Oct 03, 2019
Last update date Dec 03, 2019
Contact name Jennifer Rachelle Bermick
E-mail(s) jennifer-bermick@uiowa.edu
Organization name University of Iowa
Department Pediatrics
Lab Bermick
Street address 431 Newton Road, 505 EMRB
City Iowa City
State/province IA
ZIP/Postal code 52242
Country USA
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (41)
GSM4106243 U30_naive CD4+ T cells_H3K4me3_rep1
GSM4106244 U30_naive CD4+ T cells_H3K4me3_rep1_input
GSM4106245 U30_naive CD4+ T cells_H3K4me3_rep2
Relations
BioProject PRJNA575695

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE138378_RAW.tar 8.0 Gb (http)(custom) TAR (of BW)
Raw data are available in SRA
Processed data provided as supplementary file
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