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Series GSE138367 Query DataSets for GSE138367
Status Public on Oct 05, 2019
Title In vitro and in vivo antitumor activity of cucurbitacin C, a novel natural product from cucumber
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Cucurbitacin C (CuC), a novel analogue of triterpenoids cucurbitacins, confers a bitter taste in cucumber. Genes and signaling pathways responsive for biosynthesis of CuC have been identified in the recent years. In the present study, we explored the anti-cancer effects of CuC against human cancers in vitro and in vivo. CuC inhibited proliferation and clonogenic potential of multiple cancer cells in a dose dependent manner. Low dose CuC treatment induced cell cycle arrest at G1 or G2/M stage in different cancer lines, whereas high dose treatment of CuC caused apoptosis in cancer cells. PI3K-Akt signaling pathway was found to be one of the major pathways involved in CuC-induced cell growth arrest and apoptosis by RNA-Seq and western blotting. Mechanistic dissection further confirmed that CuC effectively inhibited the Akt signaling by inhibition of Akt phosphorylation at Ser473. In vivo CuC treatment (0.1 mg/kg body weight) effectively inhibited growth of cancer cells derived xenograft tumors in athymic nude mice and caused significant apoptosis. Our findings for the first time demonstrated the potential therapeutic significance of CuC against human cancers.
Overall design Experiment the antitumor activity of cucurbitacin C In vitro and in vivo
Contributor(s) Wu D, Wang Z, Lin M, Shang Y, Wang F, Zhou J, Wang F, Luo X, Huang W
Citation(s) 31780930
Submission date Oct 03, 2019
Last update date Jan 05, 2020
Contact name zhang xiantong
Phone 18800119079
Organization name Southern Medical University Shenzhen Hospital
Street address Xinan Road
City Shenzhen
ZIP/Postal code 518101
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (4)
GSM4106052 HepG2_20
GSM4106053 HepG2_40
GSM4106054 PC-3_20
BioProject PRJNA575644

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE138367_RAW.tar 6.0 Mb (http)(custom) TAR (of TXT)
Raw data are available in SRA
Processed data provided as supplementary file

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