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| Status |
Public on Nov 04, 2020 |
| Title |
Transcriptional response of NGN3 J2 enteroids |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Enteroendocrine cells are hormonal secreting cells in the gut. However, as they comprise <1% of the total epithelial cell population, studies on their response to stimuli have been limited. Chang-Graham, Danhof, Engevik, and Tomaro-Duchesneau et al (PMID 31029854) developed a model enteroid system to overcome this limitation. By driving expression of NGN3 in human jejunal enteroids, their system allows for analysis of hormonal secretion and transcriptional analysis in response to a stimulus. To further characterize these NGN3 enteroids, we performed RNASeq on these enteroids in the induced or uninduced state.
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| Overall design |
A total of 12 samples were sequenced. In the uninduced state (NGN3 not induced, low number of enteroendocrine cells), 2 batches of enteroids, triplicate enteroids for each treatment: media alone (LDM4) = 6 samples. In the induced state (NGN3 induced, high number of enteroendocrine cells), 2 batches of enteroids, triplicate enteroids for each treatment: media alone (LDM4) = 6 samples.
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| Contributor(s) |
Di Rienzi SC, Danhof HA, Britton RA |
| Citation(s) |
31029854 |
| Submission date |
Oct 02, 2019 |
| Last update date |
Nov 04, 2020 |
| Contact name |
Sara Chloe Di Rienzi |
| E-mail(s) |
sdirienzi@gmail.com
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| Organization name |
Baylor College of Medicine
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| Department |
Molecular Virology and Microbiology
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| Lab |
Britton
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| Street address |
1 Baylor Plaza Room 743E
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA575547 |
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