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Series GSE138187 Query DataSets for GSE138187
Status Public on Jul 25, 2022
Title Genome-wide chromatin analyses of liver cells upon YAP induction [Liver_ChIPSeq_LapTTA]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary YAP1 is a transcriptional co-activator regulated by the Hippo pathway, mechano-transduction, G-protein coupled receptors and WNT signaling. YAP1 activity depends on its stabilization and its nuclear localization. Once in the nucleus, YAP1 can regulate gene expression by interacting with transcription factors with DNA binding capability. While YAP1 main transcriptional partners belong to the TEAD family, several reports indicate that YAP1 can also associate with SMADs, RUNX and other transcription factors. YAP1 acts as a broad regulator of cell growth and differentiation, thus playing a pivotal role in regeneration, re-programming and cancer. How YAP1 may control such a large number of cellular processes is still to be fully understood.
To identify how YAP1 controls transcription and to define gene expression programs directly regulated by YAP1, we conducted a genome wide investigation in the mouse liver. Integrated analyses of ChIP-seq and RNA-seq data indicated that YAP1 can act both as an activator and as a repressor in a context dependent way, and allowed the dissection of transcriptional mechanisms. Longitudinal analysis by ChIP-seq and RNA-seq highlighted differences in genome binding and transcriptional control which allowed the identification of acute and tonic YAP1 dependent responses. While during acute activation YAP1 supported cell cycle and signal transduction programs, its prolonged activation sustained a feedforward loop that potentiated and expanded YAP1 dependent transcription thus leading to the direct engagement of cell identity programs.
Overall this study provides a genome scale understanding of how YAP1 controls gene expression and reveals principles in gene regulation which account for YAP1 ability to orchestrate development, regeneration and cell reprogramming.
Overall design ChIPseq and ATAC seq analyses were performed on liver of LaptTA-WT (wild type control) or LaptTA-tetYAP mice activated for 4 weeks (4W)

Please note that the processed data generated from all replicates is linked to the corresponding 'rep 1' sample records and indicated in the associated sample description field.
Contributor(s) Campaner S, De Fazio S, Biagioni F, Croci O
Citation(s) 35871292
Submission date Sep 30, 2019
Last update date Jul 26, 2022
Contact name stefano campaner
Organization name fondazione Istituto italiano di tecnologia
Department Center for Genomic Science
Lab Cancer Biology
Street address via adamello 16
City milano
ZIP/Postal code 20139
Country Italy
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (27)
GSM4101459 Liver_ChIPSeq_Yap_LapTTA_WT
GSM4101460 Liver_ChIPSeq_Brd4_LapTTA_WT_rep1
GSM4101461 Liver_ChIPSeq_Brd4_LapTTA_WT_rep2
This SubSeries is part of SuperSeries:
GSE138191 Genome-wide chromatin analyses and RNA-seq of liver cells upon YAP induction
BioProject PRJNA575010
SRA SRP223748

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Supplementary file Size Download File type/resource
GSE138187_RAW.tar 3.6 Gb (http)(custom) TAR (of BED, BW, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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