GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE138143 Query DataSets for GSE138143
Status Public on Jan 14, 2021
Title Hnf4a regulated transcriptome in 2D cell lines derived from murine pancreatic tumors [2D cell lines]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Pancreatic ductal adenocarcinoma is an aggressive disease with a dismal five-year survival of 5%. Gene expression profiling has been instrumental for subtype classification in cancer, highlighting fundamental differences in tumors at the molecular level. Over the last years, multiple genomics studies have led to the classification of PDAC into two major subtypes: classical and basal-type. The classical subtype expresses higher levels of endodermal lineage specifiers, including HNF4A, GATA6, FOXA2, FOXA3 than the basal-type. The basal-type confers a worse prognosis, raising the possibility that loss of these lineage specifiers might enhance the malignant potential of PDAC. We found that the lineage specifier HNF4a plays a key role in maintaining a transcriptional network that characterizes the classical subtype, restraining growth in different PDAC models. Additionally, we demonstrated that HNF4a controls PDAC cell identity and proliferation, and represses the expression of SIX family members, two mesodermal lineage specifiers highly expressed in basal-type.
Overall design The study was designed to compare transcriptome profiles between isogenic Hnf4a-negative and Hnf4a-expressing murine pancreatic cancer cell lines. Hnf4a expression was restored by treating cell cultures with doxycycline.
Contributor(s) Camolotto SA, Belova VK, Torre-Healy L, Conway H, Stubben C, Moffit R
Citation(s) 32826305
Submission date Sep 30, 2019
Last update date May 16, 2022
Contact name Eric Snyder
Organization name University of Utah, Huntsman Cancer Institute
Department Pathology
Street address 2000 Circle of Hope Drive
City Salt Lake City
State/province UT
ZIP/Postal code 84103
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM4100940 15145X7 HC800-A8-1-Control rep1
GSM4100941 15145X8 HC800-A8-1-DOX rep1
GSM4100942 15783X1 HC569-A8-18-Control rep1
This SubSeries is part of SuperSeries:
GSE138145 Hnf4a regulated transcriptome
BioProject PRJNA574923
SRA SRP223682

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE138143_counts_2D.txt.gz 433.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap