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Series GSE138142 Query DataSets for GSE138142
Status Public on Jan 14, 2021
Title Hnf4a regulated transcriptome in organoid cultures derived from murine pancreas where HNnf4a is deleted after tumor initiation. [organoid]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Pancreatic ductal adenocarcinoma is an aggressive disease with a dismal five-year survival of 5%. Gene expression profiling has been instrumental for subtype classification in cancer, highlighting fundamental differences in tumors at the molecular level. Over the last years, multiple genomics studies have led to the classification of PDAC into two major subtypes: classical and basal-type. The classical subtype expresses higher levels of endodermal lineage specifiers, including HNF4A, GATA6, FOXA2, FOXA3 than the basal-type. The basal-type confers a worse prognosis, raising the possibility that loss of these lineage specifiers might enhance the malignant potential of PDAC. We found that the lineage specifier HNF4a plays a key role in maintaining a transcriptional network that characterizes the classical subtype, restraining growth in different PDAC models. Additionally, we demonstrated that HNF4a controls PDAC cell identity and proliferation, and represses the expression of SIX family members, two mesodermal lineage specifiers highly expressed in basal-type.
Overall design The study was designed to compare transcriptome profiles between isogenic Hnf4a-positive and Hnf4a-negative murine pancreatic cancer organoid lines. Hnf4a was deleted by treating the 3D cultures with 4OH-Tamoxifen
Contributor(s) Camolotto SA, Belova VK, Torre-Healy L, Stubben C, Moffit R, Snyder EL
Citation(s) 32826305
Submission date Sep 30, 2019
Last update date May 16, 2022
Contact name Eric Snyder
Organization name University of Utah, Huntsman Cancer Institute
Department Pathology
Street address 2000 Circle of Hope Drive
City Salt Lake City
State/province UT
ZIP/Postal code 84103
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM4100932 15143X1 SC1640-control
GSM4100933 15143X2 SC1640-4-OH-Tam
GSM4100934 15143X3 SC1693-control
This SubSeries is part of SuperSeries:
GSE138145 Hnf4a regulated transcriptome
BioProject PRJNA574922
SRA SRP223681

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Supplementary file Size Download File type/resource
GSE138142_counts_organoid.txt.gz 437.9 Kb (ftp)(http) TXT
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Processed data are available on Series record

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