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Series GSE138112 Query DataSets for GSE138112
Status Public on Oct 01, 2021
Title Genome-wide analysis of m6A modification
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary N6-methyladenosine (m6A) might exert stronger affection on circRNA expression, beyond linear genes.We confirmed the view by analysing total RNA meRIP-Seq from GSE114150 and three other sets of data using RNA sequencing. Here, we study the difference in circRNA levels between wild (WT) and experimental (D395A-mutation; METTL3-knock-out) groups when m6A level changes. Results reveal knockout and D395A mutation of METTL3 could both increase the circRNA expression and the ratio of circular-to-linear. Our data propose a new pattern of circRNAs development and expand the understanding of m6A functions.
 
Overall design mettl3 is knockedout using Crispr in embryonic stem cell in order to study the interaction between m(6)A and circRNA.
 
Contributor(s) Xiao S, Han Y
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Submission date Sep 27, 2019
Last update date Oct 02, 2021
Contact name Shan Zhong
E-mail(s) zhongshan@whu.edu.cn
Organization name Wuhan University
Street address Donghu Road 185
City Wuhan
ZIP/Postal code 430071
Country China
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (6)
GSM4100348 WT-1
GSM4100349 WT-2
GSM4100350 D395A-mutation-1
Relations
BioProject PRJNA574639
SRA SRP223528

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE138112_RAW.tar 630.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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