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Series GSE137942 Query DataSets for GSE137942
Status Public on Sep 25, 2019
Title Genome-wide maps of chromatin state in 5-HT6R knock out mice.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose:The primary cilia play central roles in transducing or regulating various signaling pathways during brain development. However, the function of primary cilia in mature nerve system is currently largely unknown.We aimed to identify transcriptional changes resulting from depletion of ciliary GPCR 5-HT6R.
Methods: We performed RNA sequencing in the hippocampal tissues from P90 adult konck out mice and wild type mice.
Results:We examined for differential expression genes across 27698 genes expressed in our datasets. At significant cutoffs corresponding to Benjamini and Hochberg adjusted p value < 0.05, we found 429 differential expression genes in 5-HT6R-/- mice compared with WT litter mates.
Conclusions:We discovered that primary cilia were associated with axon initial segment (AIS) morphology, including AIS length and position. Our data strongly suggest that the cilia signaling might have a remarked impact on neuronal excitability by regulating AIS morphology and are likely to be associated with neuronal system diseases.
Overall design Hippocampus mRNA profiles of 90-day old wild type (WT) and 5-HT6R-/- mice were generated by deep sequencing, in triplicate.
Contributor(s) Wang B, Sun Z
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Submission date Sep 24, 2019
Last update date Sep 26, 2019
Contact name Bingjie Wang
Organization name Peking University
Street address Yiheyuan ROAD 5
City Beijing
ZIP/Postal code 100089
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM4094682 WT1
GSM4094683 WT2
GSM4094684 WT3
BioProject PRJNA573916
SRA SRP223109

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Supplementary file Size Download File type/resource
GSE137942_genes.fpkm.xlsx 4.8 Mb (ftp)(http) XLSX
GSE137942_genes.rawcount.xlsx 2.0 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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