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Series GSE137840 Query DataSets for GSE137840
Status Public on Feb 18, 2020
Title Liver high-throughput sequencing of high-fat C57BL/6J wild-type mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Eight weeks after C57BL / 6J mice fed a high-fat diet (HFD), the degree of insulin resistance was assessed by intraperitoneal glucose tolerance test (IPGTT) and the insulin resistance model was successfully established. Mice were then treated with resveratrol and metformin for 6 weeks and blood and liver tissue samples were collected. Blood biochemical parameters were determined by kit, protein expression of hepatic insulin signaling pathway mRNA and mRNA was determined by Western blot and real-time polymerase chain reaction, and histomorphological changes were observed by histological staining. Mouse liver tissue samples were detected by high-throughput sequencing technology to obtain differential lncRNA expression profiles. Further differentially expressed genes were analyzed using bioinformatics techniques.
Overall design Mice were divided into 4 groups, the livers were removed, snap frozen on dry ice, and RNA was harvested using Trizol reagent. Clusters were generated by cBot, diluted to a 10 pM library, and then sequenced on an Illumina NovaSeq 6000 (Illumina, USA).
Contributor(s) Shu L
Citation(s) 32051733, 32627012, 34581420
Submission date Sep 23, 2019
Last update date Oct 13, 2021
Contact name Linyi Shu
Organization name Hebei General Hospital
Department Department of Endocrinology
Street address 348 Heping West Road
City Shijiazhuang
State/province Hebei
ZIP/Postal code 050000
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (16)
GSM4089375 C1-1L
GSM4089376 C1-4L
GSM4089377 C1-5L
This SubSeries is part of SuperSeries:
GSE178474 Whole transcriptome analysis of resveratrol and metformin improving high fat diet induced insulin resistance in liver and muscle
BioProject PRJNA573644
SRA SRP222955

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE137840_RAW.tar 16.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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