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Series GSE137825 Query DataSets for GSE137825
Status Public on Sep 23, 2019
Title Circular RNAs expression profiles in canine mammary tumours
Organism Canis lupus familiaris
Experiment type Expression profiling by high throughput sequencing
Summary We explored the expression profile of circRNAs in canine mammary tumours using high-throughput sequencing technology. In our study, we analysed the expression profiles of 3 pairs of canine mammary tumours and their adjacent normal tissues. The total RNA was extracted, and a RNA library was constructed. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses revealed that these genes were mainly concentrated in 14 biological pathways. We selected 11 validated circRNAs and further confirmed the existence of these circRNAs by qRT-PCR. The circRNA-miRNA network diagram was constructed by using Cytoscape software. We found a total of 14851 circRNAs and 106 differentially expressed circRNAs in canine mammary tumours and their adjacent normal tissues (fold change ≥ 2, P ≤ 0.05). There were 64 upregulated circRNAs and 42 downregulated circRNAs. The main GO functions were the regulation of the regulated secretory pathway, the regulation of neurotransmitter secretion and the positive regulation of phagocytosis. Most of these pathways were related to the cGMP-PKG (cyclic guanosine monophosphate) signalling pathway, the cAMP (cyclic adenosine monophosphate) signalling pathway and the OXYTOCIN signalling pathway. CircRNAs have the function of adsorbing miRNA, similar to a sponge, and we further constructed the interaction network of circRNAs and miRNAs. The screened source genes closely related to canine mammary tumours included RYR2, PDE4D, ROCK2, CREB3L2 and UBA3. The screened circRNAs related to canine mammary tumours included chr27:26618544-26687235-, chr26:8194880-8201833+, and chr17:7960861-7967766-.In conclusion, our study uncovered circRNA expression profiles in canine mammary tumours. Moreover, some circRNAs may be used as potential biomarkers for screening dogs with high-risk canine mammary tumours.
 
Overall design Examination of the circRNAs of 3 pairs of canine mammary tumours and their adjacent normal tissues.
 
Contributor(s) Chen H, Li H
Citation(s) 35622733
Submission date Sep 22, 2019
Last update date Jun 21, 2022
Contact name Kun Jia
Organization name South China Agricultural University
Department College of Veterinary Medicine
Street address Wushan Street
City Guangzhou
ZIP/Postal code 510642
Country China
 
Platforms (1)
GPL24229 Illumina HiSeq 4000 (Canis lupus familiaris)
Samples (6)
GSM4089061 canine mammary tumour tissue [B1]
GSM4089062 canine mammary tumour tissue's adjacent normal tissue [B2]
GSM4089063 canine mammary tumour tissue [D1]
Relations
BioProject PRJNA573536
SRA SRP222903

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE137825_CircRNA_Expression_Profiling.xlsx 2.2 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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