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Series GSE137800 Query DataSets for GSE137800
Status Public on Jul 15, 2020
Title Effect of ionizing radiation on transcriptome during neural differentiation of hESC
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Differentiation of hESCs to neural lineages was used as a model for early embryonic brain development in order to assess the effect of their exposure to low (17 mGy) and high (572 mGy) doses of radiation on gene expression.
Exposure of hESC to the low dose did not result in changes in gene expression at any of the time points, whereas exposure to the high dose resulted in downregulation of some major neurodifferentiation markers on days 6 and 10. Gene ontology analysis showed that pathways related to nervous system development, neurogenesis, and generation of neurons were among the most affected.
Conclusion: exposure to a low dose of 17 mGy was well tolerated by hESCs while exposure to 572 mGy significantly affected their genetic reprogramming into neuronal lineages.
Overall design Transcriptomes were assessed by RNA sequencing during neural differentiation at three time points in control and irradiated samples in three biological replicates. The first time point was when the cells were still pluripotent (day 0), the second time point was during the stage of embryoid body formation (day 6), and the last time point was during the stage of neural rosette formation (day 10).
Contributor(s) Panyutin I
Citation(s) 32216708
Submission date Sep 20, 2019
Last update date Jul 15, 2020
Contact name Igor Panyutin
Phone 3014968308
Organization name NIH/CC
Department Radiology Imaging Sciences
Lab Nuclear Medicien
Street address 10 Center DR., room 1C401
City Bethesda
State/province MD
ZIP/Postal code 20854
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (26)
GSM4088736 A00_2
GSM4088737 A00_3
GSM4088738 A00_1
BioProject PRJNA573089
SRA SRP222829

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Supplementary file Size Download File type/resource
GSE137800_fpkm_genename.txt.gz 5.9 Mb (ftp)(http) TXT
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