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| Status |
Public on Sep 17, 2022 |
| Title |
Sel1L-Hrd1 ERAD is required for the maintainenance of beta cell identity and function |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Mice with Sel1L deficient beta cells develop severe hyperglycemia, glucose intolerance and impaired insulin secretion. Using single cell sequencing, we identified that Sel1L plays a major role in maintaining beta cell maturation by suppressing TGF-beta signaling.
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| Overall design |
Islets were isolated from 5 weeks old Sel1Lf/f and Sel1LIns1 mice and following overnight recovery, trypsinized to obtain single cell suspension and processed using 10X Genomics CHROMIUM single cell 3' solution.
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| Contributor(s) |
Shrestha N, Qi L |
| Citation missing |
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| |
| Submission date |
Sep 20, 2019 |
| Last update date |
Sep 19, 2022 |
| Contact name |
Neha Shrestha |
| E-mail(s) |
nsstha@med.umich.edu
|
| Organization name |
University of Michigan
|
| Street address |
1000 Wall Street
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48195 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA573076 |
| SRA |
SRP222820 |
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