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Series GSE137697 Query DataSets for GSE137697
Status Public on Sep 19, 2022
Title Targeting CDK12-mediated transcription regulation in anaplastic thyroid carcinoma [RNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Anaplastic thyroid carcinoma (ATC) is the most aggressive type of thyroid cancer, with no effective treatment available. Identification of new anti-ATC drugs represents an urgent need. In this study, we find that ATC cells are highly sensitive to THZ531, a potent inhibitor of the transcriptional cyclin-dependent kinase (CDK), CDK12. Cell-based assays demonstrate that CDK12 inhibition significantly impedes cell cycle progression, induces apoptotic cell death, and impairs colony formation in ATC cells. THZ531 causes a loss of elongating RNA polymerase II and suppresses gene expression in ATC cells. An integrative analysis of gene expression profiles and super-enhancer landscape, combining with functional assays, leads to the discovery of two new ATC cancer genes, ZC3H4 and NEMP1. Furthermore, CDK12 inhibition enhances the sensitivity of ATC cells to doxorubicin-mediated chemotherapy. Thus, these findings indicate that CDK12 is a potential therapeutic target for ATC treatment and its inhibition may help to overcome the chemoresistance in patients with ATC
Overall design mRNA profiles of CAL-62 treated with DMSO or THZ531 for 6 h were generated by deep sequencing, in duplicate, using Illumina NovaSeq.
Contributor(s) Geng M, Cao X, Zhang L
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Submission date Sep 19, 2019
Last update date Sep 20, 2022
Contact name Meijuan Geng
Organization name Tianjin Medical Universuty
Street address No.22 Qixiangtai Road
City Tianjin
ZIP/Postal code 300070
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (6)
GSM4085080 CAL-62_DMSO rep1
GSM4085081 CAL-62_DMSO rep2
GSM4085082 CAL-62_100 nM THZ531 rep1
This SubSeries is part of SuperSeries:
GSE137698 Targeting CDK12-mediated transcription regulation in anaplastic thyroid carcinoma
BioProject PRJNA566341
SRA SRP222490

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE137697_RAW.tar 610.0 Kb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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