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Series GSE137696 Query DataSets for GSE137696
Status Public on Sep 19, 2022
Title Targeting CDK12-mediated transcription regulation in anaplastic thyroid carcinoma [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Anaplastic thyroid carcinoma (ATC) is the most aggressive type of thyroid cancer, with no effective treatment available. Identification of new anti-ATC drugs represents an urgent need. In this study, we find that ATC cells are highly sensitive to THZ531, a potent inhibitor of the transcriptional cyclin-dependent kinase (CDK), CDK12. Cell-based assays demonstrate that CDK12 inhibition significantly impedes cell cycle progression, induces apoptotic cell death, and impairs colony formation in ATC cells. THZ531 causes a loss of elongating RNA polymerase II and suppresses gene expression in ATC cells. An integrative analysis of gene expression profiles and super-enhancer landscape, combining with functional assays, leads to the discovery of two new ATC cancer genes, ZC3H4 and NEMP1. Furthermore, CDK12 inhibition enhances the sensitivity of ATC cells to doxorubicin-mediated chemotherapy. Thus, these findings indicate that CDK12 is a potential therapeutic target for ATC treatment and its inhibition may help to overcome the chemoresistance in patients with ATC
 
Overall design We performed a genome-wide analysis of pSer2 signal by chromatin immunoprecipitation coupled with sequencing (ChIP–Seq).
 
Contributor(s) Geng M, Cao X, Zhang L
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Submission date Sep 19, 2019
Last update date Sep 20, 2022
Contact name Meijuan Geng
E-mail(s) gengmeijuan@tmu.edu.cn
Organization name Tianjin Medical Universuty
Street address No.22 Qixiangtai Road
City Tianjin
ZIP/Postal code 300070
Country China
 
Platforms (1)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (6)
GSM4085074 DMSO pSer2 Pol II ChIP
GSM4085075 DMSO pSer2 Pol II ChIP_input
GSM4085076 100 pSer2 Pol II ChIP
This SubSeries is part of SuperSeries:
GSE137698 Targeting CDK12-mediated transcription regulation in anaplastic thyroid carcinoma
Relations
BioProject PRJNA566340
SRA SRP222489

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE137696_RAW.tar 223.0 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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