GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE137597 Query DataSets for GSE137597
Status Public on Sep 17, 2020
Title Single cell sequencing of mice brain after stereotactic injection treatment
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We report the application of single cell sequencing technology for high-throughput profiling in mice brain cells.In order to search for SCZ-related molecular pathways in different brain cell types affected by immune activation, we first activated the peripheral immune system by intraperitoneal injection (IPI) of lipopolysaccharide (LPS) into adolescent mice twice on post-natal day 45 and 46, and collected the serum from the mice on the third day. Then we performed intracerebroventricular injection (ICVI) of 15 ul of serum from the immune-activated mice. After ICVI of immune-activated serum for one week, we carried out single-cell transcriptome sequencing on the brains from the mice. This study provides characterization of diverse cell populations towards different treatments.
Overall design Examination of 2 treatment effects on brain cells in mice.
Contributor(s) Gao Y, Liang X, Li Y, Li S, Zhang B, Yang X
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Sep 17, 2019
Last update date Sep 19, 2020
Contact name Xinping Yang
Phone 02062786524
Organization name Southern Medical University
Lab Xinping Yang
Street address 1838 N. Guangzhou Ave
City Guangzhou
State/province Guangdong
ZIP/Postal code 510515
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM4082883 saline-serum-injection
GSM4082884 LPS-serum-injection
BioProject PRJNA565984
SRA SRP222090

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE137597_RAW.tar 97.3 Mb (http)(custom) TAR (of TAR)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap