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| Status |
Public on Sep 17, 2020 |
| Title |
Single cell sequencing of mice brain after stereotactic injection treatment |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report the application of single cell sequencing technology for high-throughput profiling in mice brain cells.In order to search for SCZ-related molecular pathways in different brain cell types affected by immune activation, we first activated the peripheral immune system by intraperitoneal injection (IPI) of lipopolysaccharide (LPS) into adolescent mice twice on post-natal day 45 and 46, and collected the serum from the mice on the third day. Then we performed intracerebroventricular injection (ICVI) of 15 ul of serum from the immune-activated mice. After ICVI of immune-activated serum for one week, we carried out single-cell transcriptome sequencing on the brains from the mice. This study provides characterization of diverse cell populations towards different treatments.
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| Overall design |
Examination of 2 treatment effects on brain cells in mice.
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| Contributor(s) |
Gao Y, Liang X, Li Y, Li S, Zhang B, Yang X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 17, 2019 |
| Last update date |
Sep 19, 2020 |
| Contact name |
Xinping Yang |
| E-mail(s) |
xpyang1@smu.edu.cn
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| Phone |
02062786524
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| Organization name |
Southern Medical University
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| Lab |
Xinping Yang
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| Street address |
1838 N. Guangzhou Ave
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| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510515 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA565984 |
| SRA |
SRP222090 |
