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Series GSE137538 Query DataSets for GSE137538
Status Public on May 27, 2020
Title RNA sequencing of different neutrophil subpopulations from bone marrow
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The purpose of this study is to learn the dynamic transcriptomic and molecular changes during neutrophil development. We investigated the gene expression profiles of purified morphology-defined neutrophil populations. Promyelocytes (PMs), myelocytes (MCs), metamyelocytes (MMs), band neutrophils and segmented neutrophils (BN/SNs) were isolated by FACS based on differential expression of C-Kit and Ly6g.Their identities were confirmed by morphological examination. Bulk RNA sequencing was then performed to reveal the gene expression profiles and molecular signatures of each cell population. Detailed analysis of gene expression revealed that MBs highly expressed the stem cell marker Cd34 and translation-related genes such as Eef1a1, while the highest expression of primary granule-related genes such as Mpo, Elane, Prtn3, and Cstg was detected in the PM population. MCs and MMs were highly proliferative neutrophils expressing high levels of cell cycle-related genes which were significantly downregulated in mature neutrophils. Finally, this study provides a framework for the detail study of the neutrophil development and for the future application in the modulation of the granulopoiesis under pathological conditions.
Overall design Five neutrophil populations: promyelocytes (PMs), myelocytes (MCs), metamyelocytes (MMs), band neutrophils and segmented neutrophils (BN/SNs) were isolated by FACS based on differential expression of C-Kit and Ly6g.
Contributor(s) Zhang X, Xie X, Shi Q
Citation(s) 32719519, 38195694
Submission date Sep 16, 2019
Last update date Jan 18, 2024
Contact name Xuemei Xie
Organization name Boston Children's Hospital
Department Transfusion Medicine
Lab Luo's Lab
Street address 811 Enders Building, 320 Longwood Ave.
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (15)
GSM4081530 20170926_BM_NEU1_XZ4704-1_S13
GSM4081531 20170926_BM_NEU2_XZ4704-1_S14
GSM4081532 20170926_BM_NEU3_XZ4704-1_S15
This SubSeries is part of SuperSeries:
GSE137540 Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection
BioProject PRJNA565799
SRA SRP221813

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Supplementary file Size Download File type/resource
GSE137538_2019Granulocyte_bulk_matrix.csv.gz 504.0 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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