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Series GSE137519 Query DataSets for GSE137519
Status Public on Sep 17, 2019
Title RNA-Seq reveals novel mechanistic targets of withaferin A (WA) in prostate cancer cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary RNA-Seq analysis using WA-treated 22Rv1 human prostate cancer cells revealed upregulation and downregulation of 6993 and 6607 genes, respectively when compared to the vehicle-treated control. The pathways with significantly upregulated gene expression following WA treatment included mitogen-activated protein kinases, focal adhesion, endocytosis, and autophagy as revealed by KEGG and gene ontology analyses. WA treatment also resulted in downregulation of genes associated with metabolic pathways, oxidative phosphorylation, tricarboxylic acid cycle, cell cycle, and base excision repair. This study identifies novel mechanistic targets of WA in prostate cancer.
Overall design Examination of altered genes expression in 22Rv1 human prostate cancer cell line after 16-h treatment with vehicle-treated control (n=3) or 2 ┬ÁM of WA (n=3)
Contributor(s) Singh SV
Citation(s) 32002539
Submission date Sep 16, 2019
Last update date Mar 11, 2020
Contact name Shivendra V Singh
Phone 412-623-3263
Organization name University of Pittsburgh
Department Pharmacology and Chemical Biology
Lab Singh
Street address 5117 Centre Avenue UPMC HCC Research Pavilion Suite 2.32A
City Pittsburgh
State/province PA
ZIP/Postal code 15213
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (6)
GSM4080845 EV_0_1
GSM4080846 EV_0_2
GSM4080847 EV_0_3
BioProject PRJNA565770
SRA SRP221766

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Supplementary file Size Download File type/resource
GSE137519_DEG.txt.gz 3.1 Mb (ftp)(http) TXT
GSE137519_FPKM.txt.gz 982.4 Kb (ftp)(http) TXT
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