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Series GSE137507 Query DataSets for GSE137507
Status Public on Jan 27, 2021
Title Transcriptomic profiling of wild-type C2C12 cells and C2C12 cells with Myod1 gene knockout by RNA-sequencing
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary C2C12 is a myoblast cell line usually used for study of muscle differentiation. Myod1 is a pro-differentiation factor for myogenesis. We found that CRISPR/Cas9-mediated Myod1 gene knockout in C2C12 cells led to the loss of myoblast identity and gain of neural properties.
Overall design Myod1 gene was knocked out with CRISPR/Cas9. Wild type C2C12 cells (C2C12(WT)) and gene knockout cells (C2C12(Myod1-/-)) showed different phenotypes when cultured in serum-free medium. Each three samples of C2C12(WT) and C2C12(Myod1-/-) were collected and subjected to RNA-sequencing.
Contributor(s) Cao Y, Zhang M
Citation(s) 33468253
Submission date Sep 16, 2019
Last update date Jan 27, 2021
Contact name Ying Cao
Organization name Nanjing University
Street address 12 Xuefu Rd., Pukou High-Tech Zone
City Nanjing
ZIP/Postal code 210061
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM4080743 C2C12(WT) rep1
GSM4080744 C2C12(WT) rep2
GSM4080745 C2C12(WT) rep3
BioProject PRJNA565753
SRA SRP221751

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Supplementary file Size Download File type/resource
GSE137507_genes.fpkm.anno.xlsx 3.0 Mb (ftp)(http) XLSX
GSE137507_trans.fpkm.anno.xlsx 8.7 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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