GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE137454 Query DataSets for GSE137454
Status Public on Jul 29, 2020
Title Long noncoding RNA INCA1 regulates tumor interferon signaling
Organism Homo sapiens
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Tumor interferon signaling regulates the expression of immunosuppressive molecules and promotes cancer immune evasion. Although the role of long noncoding RNAs (lncRNAs) in the regulation of gene expression is now emerging, their function in tumor interferon signaling remains unexplored. We have identified the interferon-γ (IFNγ)-stimulated non-coding RNA 1 (INCA1) as a novel lncRNA expressed form the PD-L1 locus. INCA1 is expressed in multiple tumor types and its levels increase after IFNγ stimulation. In this study we performed transcriptome analysis (RNA-seq) of INCA1 knockdown cells and show that INCA1 regulates the expression of several interferon-stimulated genes. Overall, our findings reveal INCA1 as a critical component of the tumor interferon signaling.
Overall design Tumor cell lines stable expressing shRNA control or shRNA targeting INCA1 were stimulated with interferon gamma for 24 hours. RNA expression profiles were generated by RNA-seq, in triplicate, using Illumina NovaSeq6000.
Contributor(s) Mineo M, Chiocca EA
Citation(s) 32504554
Submission date Sep 15, 2019
Last update date Jul 29, 2020
Contact name Marco Mineo
Phone 6175255656
Organization name Brigham and Women's Hospital
Street address 60 Fenwood Rd, BTM 8012
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (12)
GSM4079644 shCTRL-C1
GSM4079645 shCTRL-C2
GSM4079646 shCTRL-C3
This SubSeries is part of SuperSeries:
GSE137489 Long noncoding RNA INCA1 regulates tumor interferon signaling.
BioProject PRJNA565585
SRA SRP221667

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE137454_INCA1_KD.txt.gz 513.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap