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Status |
Public on Jul 29, 2020 |
Title |
Long noncoding RNA expression profiles analysis in GBM patient-derived cells stimulated with interferon gamma |
Organism |
Homo sapiens |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
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Summary |
Long noncoding RNAs (lncRNAs) are key regulators of gene expression both in physiological and pathological conditions. Previous studies showed changes in lncRNA expression during tumorigenesis. To analyze the role of lncRNAs in tumor interferon signaling and cancer-mediated immunosuppression, we used RNA-seq technique to identify lncRNAs that are differentially expressed in GBM patient-derived cells stimulated with interferon gamma compared to unstimulated cells. 15,768 lncRNAs were surveyed in the analysis. RNA-seq data show upregulation of 113 lncRNAs upon interferon gamma treatment (p value < 0.01, fold change > 2). Our findings indicate that the expression of several lncRNAs is stimulated by interferon gamma suggesting a critical role of lncRNAs in tumor interferon signaling.
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Overall design |
Patient-derived GBM cells were stimulated with interferon gamma for 24 hours. Non stimulated cells were used as control. LncRNA expression profiles were generated by RNA-seq, in triplicate, using Illumina HiSeq 4000.
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Contributor(s) |
Mineo M, Chiocca EA |
Citation(s) |
32504554 |
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Submission date |
Sep 15, 2019 |
Last update date |
Jul 29, 2020 |
Contact name |
Marco Mineo |
E-mail(s) |
mmineo@bwh.harvard.edu
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Phone |
6175255656
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Organization name |
Brigham and Women's Hospital
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Street address |
60 Fenwood Rd, BTM 8012
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City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
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Platforms (1) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (6)
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This SubSeries is part of SuperSeries: |
GSE137489 |
Long noncoding RNA INCA1 regulates tumor interferon signaling. |
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Relations |
BioProject |
PRJNA565586 |
SRA |
SRP221666 |