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Series GSE137449 Query DataSets for GSE137449
Status Public on Dec 31, 2019
Title Role of Cyp2b in diet-induced nonalcoholic steatohepatitis (NASH)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To investigate the role of hepatic CYP2B in diet-induced nonalcoholic steatohepatitis (NASH), a Cyp2b triple knockout mouse lacking Cyp2b9, Cyp2b10, and Cyp2b13 was developed using CRISPER/Cas9. Wildtype (WT) and Cyp2b-null mice were fed a normal diet (ND) or a choline-deficient, L-amino acid-defined high-fat diet (CDAHFD), containing 0.1% methionine and 62% fat for 8 weeks. RNA was extracted from the livers of female and male mice from all treatment groups and used for RNA seqencing. RNAseq data demonstrated that a lack of Cyp2b was protective in female but more harmful in male mice. Hepatic gene expression revealed a higher number of phase I-III xenobiotic metabolism and inflammatory response genes were down-regulated in CDAHFD-fed WT female and Cyp2b-null male mice.
Overall design Use RNA-sequencing to investigate the role of Cyp2b in diet-induced NASH on a transcriptomic level, by comparing the livers of WT and Cyp2b-null mice fed a CDAHFD for 8 weeks using Illumina technology.
Contributor(s) Heintz MM, Noorai RE, Baldwin WS
Citation(s) 32155178
NIH grant(s)
Grant ID Grant title Affiliation Name
R15 ES017321 Does the inhibition of key xenobiotic-metabolizing CYPs cause obesity? CLEMSON UNIVERSITY William S Baldwin
P20 GM109094 Clemson University Genomics Institute (CUGI) CLEMSON UNIVERSITY Christopher Alan Saski
Submission date Sep 14, 2019
Last update date Mar 31, 2020
Contact name William S Baldwin
Phone 915-491-7366
Organization name Clemson University
Department Biological Sciences
Lab Dr. William Baldwin
Street address 230 Parkway Dr
City Clemson
State/province South Carolina
ZIP/Postal code 29634
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (32)
GSM4079560 WT_NDF_1
GSM4079561 WT_NDF_2
GSM4079562 WT_NDF_3
BioProject PRJNA565506
SRA SRP221637

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Supplementary file Size Download File type/resource
GSE137449_Rawcounts_Females_Cyp2bnull_NASH_study.txt.gz 605.1 Kb (ftp)(http) TXT
GSE137449_Rawcounts_Males_Cyp2bnull_NASH_study.txt.gz 625.6 Kb (ftp)(http) TXT
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Processed data are available on Series record

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