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Series GSE137385 Query DataSets for GSE137385
Status Public on Mar 05, 2020
Title Nutrient regulation of liver gene expression
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We have obtained RNAseq data from the livers of male C57BL/6J and BALB/c that were maintained on a low-fat (10 kcal%fat) diet (PicoLab Mouse Diet 5053) and then fasted overnight or following fasting fed with low-fat diet supplemented with or without 20% glucose, fructose or sucrose in their drinking water for 3, 6 and 12 h. Out of 130 mice, we have successfully obtained 128 liver mRNAseq datasets that are currently being analyzed for differences in specific gene expression, the extent of gene expression and the inter-strain variability among replicates for each gene in each strain and under each dietary condition. We have observed distinct expression differences in terms of the time course and magnitude of gene expression changes between these two strains of mice. These differences also are dependent upon the specific carbohydrate present in each diet.
Overall design Male mice at 8 weeks of age were purchased from the Jackson Laboratory and trained for 1 week for a well timed fasting and feeding protocol. On the last day of training, after fasting for 21 h, mice were sacrificed at 0, 3, 6 and 12 h following feeding and their livers were isolated and snap frozen in liquid N2. Total RNA was extracted. RNAseq ~ 30 million reads, 150 bp pair-ended were obtained.
Contributor(s) Chi Y, Liu L, Pessin JE
Citation(s) 32075912, 34643091
Submission date Sep 13, 2019
Last update date Oct 21, 2021
Contact name Jeffrey E Pessin
Organization name Albert Einstein College of Medicine
Department Medicine and Molecular Pharmacology
Lab Diabetes Research Center
Street address 1301 Morris Park Avenue
City Bronx
State/province NY
ZIP/Postal code 10461
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (128)
GSM4077953 Liver, C57BL6/J, Control Water, 0h (JC001)
GSM4077954 Liver, BALB/c, Control Water, 0h (JC002)
GSM4077955 Liver, C57BL6/J, Control Water, 3h (JC003)
BioProject PRJNA565352
SRA SRP221546

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE137385_fpkm_group.csv.gz 3.9 Mb (ftp)(http) CSV
GSE137385_fpkm_sample.csv.gz 15.8 Mb (ftp)(http) CSV
GSE137385_readcount_genename.csv.gz 4.8 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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