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Series GSE137274 Query DataSets for GSE137274
Status Public on Apr 14, 2020
Title A WIZ/Cohesin/CTCF complex anchors DNA loops to define gene expression and cell identity [Hi-C]
Organism Mus musculus
Experiment type Other
Summary Purpose: Chromatin architecture is an important regulator of gene expression, but details of how DNA loops form and function remain unclear. The purpose of this study is to assess the role of Widely Interspaced Zinc-fingers protein (WIZ) in gene regulation and DNA looping.
Method: Genome wide chromatin looping patterns in the presence or absence of WIZ were assessed via Hi-C in both wildtype and CRISPR-Cas9 genome edited WIZ deletion mouse embryonic stem cells (mESCs).
Overall design Hi-C was performed in wild type and Wiz-deletion mESCs in duplicate.
Contributor(s) Dowen JM, Justice MN, Carico ZM, Stefan HC
Citation(s) 32294452
Submission date Sep 11, 2019
Last update date May 18, 2020
Contact name Jill Dowen
Organization name UNC Chapel Hill
Street address 250 Bell Tower Drive, CB 7100 3360 Genome Sciences Building
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
Platforms (2)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM4074303 WT rep1 (Hi-C)
GSM4074304 WT rep2 (Hi-C)
GSM4074305 Wiz-del rep1 (Hi-C)
This SubSeries is part of SuperSeries:
GSE137285 A WIZ/Cohesin/CTCF complex anchors DNA loops to define gene expression and cell identity
BioProject PRJNA565020
SRA SRP221396

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE137274_collisions_WT.txt.gz 4.4 Gb (ftp)(http) TXT
GSE137274_collisions_Wiz-del.txt.gz 5.4 Gb (ftp)(http) TXT
GSE137274_contact_domains_25kb_WT.bedpe.gz 163.9 Kb (ftp)(http) BEDPE
GSE137274_contact_domains_25kb_Wiz-del.bedpe.gz 161.4 Kb (ftp)(http) BEDPE
GSE137274_inter_30_WT.hic 10.2 Gb (ftp)(http) HIC
GSE137274_inter_30_Wiz-del.hic 11.0 Gb (ftp)(http) HIC
GSE137274_merged_loops_WT.bedpe.gz 194.4 Kb (ftp)(http) BEDPE
GSE137274_merged_loops_Wiz-del.bedpe.gz 256.9 Kb (ftp)(http) BEDPE
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Raw data are available in SRA
Processed data are available on Series record

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