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Series GSE137272 Query DataSets for GSE137272
Status Public on Apr 14, 2020
Title A WIZ/Cohesin/CTCF complex anchors DNA loops to define gene expression and cell identity [ChIP-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Purpose: Chromatin architecture is an important regulator of gene expression, but details of how DNA loops form and function remain unclear. The purpose of this study is to assess the role of Widely Interspaced Zinc-fingers protein (WIZ) in gene regulation and DNA looping.
Method: Genome wide binding patterns of known architectural proteins in the presence or absence of WIZ were assessed via chromatin immunoprecipitation followed by high throughput sequencing (ChIP-seq) in both wildtype and CRISPR-Cas9 genome edited WIZ deletion mouse embryonic stem cells (mESCs).
Overall design ChIP-seq was performed in wild type mESCs in duplicate for CTCF, SMC1A, RAD21, and WIZ. ChIP-seq was performed in CRISPR-Cas9 genome edited WIZ deletion mESCs in duplicate for Rad21 and CTCF.
Contributor(s) Dowen JM, Justice MN, Carico ZM, Stefan HC
Citation(s) 32294452
Submission date Sep 11, 2019
Last update date May 18, 2020
Contact name Jill Dowen
Organization name UNC Chapel Hill
Street address 250 Bell Tower Drive, CB 7100 3360 Genome Sciences Building
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
Platforms (3)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (12)
GSM4074279 CTCF Rep 1
GSM4074280 CTCF Rep 2
GSM4074281 RAD21 Rep 1
This SubSeries is part of SuperSeries:
GSE137285 A WIZ/Cohesin/CTCF complex anchors DNA loops to define gene expression and cell identity
BioProject PRJNA565019
SRA SRP221394

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Supplementary file Size Download File type/resource 505.8 Mb (ftp)(http) BW 377.8 Mb (ftp)(http) BW 574.2 Mb (ftp)(http) BW 535.1 Mb (ftp)(http) BW 506.9 Mb (ftp)(http) BW 449.3 Mb (ftp)(http) BW
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