NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE137271 Query DataSets for GSE137271
Status Public on Apr 14, 2020
Title A WIZ/Cohesin/CTCF complex anchors DNA loops to define gene expression and cell identity [RNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: Chromatin architecture is an important regulator of gene expression, but details of how DNA loops form and function remain unclear. The purpose of this study is to assess the role of Widely Interspaced Zinc-fingers protein (WIZ) in gene regulation and DNA looping.
Method: The role of WIZ in gene regulation was assessed by performing RNA-seq in both wildtype and CRISPR-Cas9 genome edited WIZ deletion mouse embryonic stem cells (mESCs).
 
Overall design RNA-seq was performed in wild type mESCs and WIZ CRISPR-Cas9 genome edited WIZ deletion mESCs in triplicate.
 
Contributor(s) Dowen JM, Justice MN
Citation(s) 32294452
Submission date Sep 11, 2019
Last update date May 18, 2020
Contact name Jill Dowen
E-mail(s) jilldowen@unc.edu
Organization name UNC Chapel Hill
Street address 250 Bell Tower Drive, CB 7100 3360 Genome Sciences Building
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (6)
GSM4074273 WT Rep 1 (RNA-Seq)
GSM4074274 WT Rep 2 (RNA-Seq)
GSM4074275 WT Rep 3 (RNA-Seq)
This SubSeries is part of SuperSeries:
GSE137285 A WIZ/Cohesin/CTCF complex anchors DNA loops to define gene expression and cell identity
Relations
BioProject PRJNA565018
SRA SRP221390

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE137271_RAW.tar 279.8 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap