GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE137244 Query DataSets for GSE137244
Status Public on Mar 17, 2021
Title Characterization of tumor mutational burden in LKB1 mutant lung tumors
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Inactivating mutations in LKB1/STK11 are present in ~20% of non-small cell lung cancers (NSCLC) and portend poor response to anti-PD-1 immunotherapy in patients and genetically engineered mouse model (GEMMs). Here, we sought to uncover the basis for immunotherapy resistance of these tumors and to define strategies that overcome this barrier. Whereas high tumor mutational burden (TMB) often correlates with response to anti-PD1 treatment, we found that LKB1-deficient NSCLCs from non-smokers and GEMMs exhibited striking elevations in nonsynonymous mutations compared to LKB1 wildtype tumors. Correspondingly, LKB1 mutant NSCLC cell lines showed defects in both replication dependent and independent double-strand DNA break (DSB) repair, which were reversed upon LKB1 re-expression.
Overall design Examine mRNA of KP and KL GEMM lung tumor nodules derived cell lines for mutational burden analysis.
Contributor(s) Deng J, Wong KK
Citation(s) 34142094
Submission date Sep 10, 2019
Last update date Jun 19, 2021
Contact name Igor Dolgalev
Organization name NYU Grossman School of Medicine
Street address 550 1st Ave
City New York
State/province New York
ZIP/Postal code 10016
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (11)
GSM4073816 B6AL10-1-RNA
GSM4073817 B6AL10-2-RNA
GSM4073818 B6AL10-3-RNA
BioProject PRJNA564852
SRA SRP221293

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE137244_counts.fpkm.csv.gz 666.2 Kb (ftp)(http) CSV
GSE137244_counts.normalized.csv.gz 850.2 Kb (ftp)(http) CSV
GSE137244_counts.raw.csv.gz 593.6 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap