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| Status |
Public on Dec 30, 2019 |
| Title |
Single cell RNA-sequencing of cardiac CD45+ populations in mouse with transverse aortic constriction (TAC) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
It is already well known that the immune cells called macrophages are involved in the mechanisms of heart failure (HF). Macrophages are divised into several subtypes based on behavior.Some subsets are highly inflammatory, while some support tissue repair. Recent studies have suggested that the classical M1/M2 classification is not suitable for some pathological processes of the heart. Single cell sequencing (scSeq) is an ideal tool to clarify the macrophage heterogeneity in HF. It provided a higher resolution of cellular differences and a better understanding of individual cell function. In this study, we aimed to identify a macrophage subset that linked with heart failure, and furtherly screen the target and investigate the mechanism.
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| Overall design |
CD45+ cells from heart of mice with/without TAC (6 per group) were sorted and the mRNA profiles of single cells were investagted by 10x Genomics technology
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| Contributor(s) |
Lu L, Shi-Hao N |
| Citation(s) |
34100920 |
| Submission date |
Sep 09, 2019 |
| Last update date |
Nov 14, 2021 |
| Contact name |
Lu Lu |
| E-mail(s) |
coinland@gzucm.edu.cn
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| Organization name |
Guangzhou University of Chinese Medicine
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| Street address |
No.12,Airport Road,Baiyun District
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| City |
Guangzhou |
| ZIP/Postal code |
510407 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA564691 |
| SRA |
SRP221182 |
