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Series GSE137165 Query DataSets for GSE137165
Status Public on Oct 16, 2019
Title Single Cell RNAseq profiles of thymic ETP populations
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The single cell profiles of sorted ETP populations with cell hashing antibody barcoding from mouse thymus
Overall design Examination of single cell expression profile of sorted populations identified from previous pseudotime analysis. Experiment was performed using droplet-based 3′ end RNA expression profiling using Chromium Single Cell 3′ Reagent Kits v3 according to manufacturer’s protocol (10x Genomics), with the FACS sorted individual sub-populations of ETPs tagged with Totalseq-A hashtags (Biolegend). The cDNA library was sequenced on Illumina Novaseq for 400 million reads, and tag library on HiSeq4000 for 20 million reads, both in pair-end mode following manufacturer's instructions.
Please note that meta.csv.gz contains assigned sample names of individual cells after demultiplexing
Contributor(s) Zhou W, Yui MA, Williams BA, Yun J, Wold BJ, Cai L, Rothenberg EV
Citation(s) 31629685
Submission date Sep 09, 2019
Last update date Jan 15, 2020
Contact name Wen Zhou
Phone 6263954915
Organization name California Institute of Technology
Department BBE
Lab Ellen Rothenberg
Street address 1200 E California Blvd, Mail Code 250-86
City Pasadena
State/province California
ZIP/Postal code 91125
Country USA
Platforms (2)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM4072329 Sorted 4 ETP subpopulations and ETP-DN2 controls
GSM4072330 Antibody sample tag library for ETP subpop
BioProject PRJNA564688
SRA SRP221183

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE137165_RAW.tar 91.9 Mb (http)(custom) TAR (of CSV, MTX, TSV)
GSE137165_meta.csv.gz 44.2 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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