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Series GSE13714 Query DataSets for GSE13714
Status Public on Jan 01, 2009
Title HOXA9 is required for survival in human MLL rearranged acute leukemias
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Leukemias that harbor translocations involving the mixed lineage leukemia gene (MLL) possess unique biological characteristics and often have an unfavorable prognosis. Gene expression analyses demonstrate a distinct profile for MLL-rearranged leukemias with consistent high-level expression of select Homeobox genes including HOXA9. Here, we investigated the effects of HOXA9 suppression in MLL-rearranged and MLL-germline leukemias utilizing RNAi. Gene expression profiling after HOXA9 suppression demonstrated co-downregulation of a program highly expressed in human MLL-AML (this study) and murine MLL-leukemia (Krivtsov et al. 2006) stem cells including HOXA10, MEIS1, PBX3 and MEF2C. Our data indicates an important role for HOXA9 in human MLL-rearranged leukemias, and suggests targeting HOXA9 or downstream programs may be a novel therapeutic option.
Overall design RNA was purified from t(9;11) MOLM-14 AML cells 44h after transduction in triplicates with 2 of the two most effective HOXA9shRNA constructs (3 x 1F3-HOXA9shRNA; 3 x 2A5-HOXA9shRNA) or GFP-controlshRNA (6x).
Contributor(s) Faber J, Krivtsov AV, Stubbs MC, Wright R, Davis TN, van den Heuvel-Eibrink M, Zwaan CM, Kung AL, Armstrong SA
Citation(s) 19056693
Submission date Nov 24, 2008
Last update date Dec 06, 2018
Contact name Joerg Faber
Organization name Children's Hospital Boston
Street address 1 Blackfan Circle
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (12)
GSM344801 MOLM14_1F3HOXA9sh_suppression_rep1
GSM344802 MOLM14_1F3HOXA9sh_suppression_rep2
GSM344803 MOLM14_1F3HOXA9sh_suppression_rep3
BioProject PRJNA110389

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Supplementary file Size Download File type/resource
GSE13714_RAW.tar 20.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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