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| Status |
Public on Apr 27, 2020 |
| Title |
Developmental trajectory of pre-hematopoietic stem cell formation from endothelium (scRNA-seq data set) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To examine the cell and molecular transitions between endothelial (E), hemogenic endothelial (HE), and intra-arterial clusters (IAC) cells, and the heterogeneity of hematopoietic stem and progenitor cells (HSPCs) within the IACs, we profiled ~40,000 cells from the caudal arteries (dorsal aorta, umbilical, vitelline arteries) of embryonic day (E) 9.5 to 11.5 mouse embryos by single-cell RNA sequencing (scRNA-Seq). We identified a developmental bottleneck in endothelial to hematopoietic transition (EHT) and heterogeneity within the IAC population.
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| Overall design |
Single cell RNA-Seq profiles of cells involved in endothelial to hematopoietic transition (EHT) from mouse embryos at embryonic day 9.5, 10.5, 11.5 and functional HSCs at E14.5.
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| Contributor(s) |
Tan K, Speck NA |
| Citation(s) |
32392346 |
| |
| Submission date |
Sep 09, 2019 |
| Last update date |
Jun 30, 2020 |
| Contact name |
Qin Zhu |
| Organization name |
UCSF
|
| Street address |
600 Minnesota Street
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94107 |
| Country |
USA |
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| Platforms (2) |
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| Samples (26)
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| This SubSeries is part of SuperSeries: |
| GSE137117 |
Developmental trajectory of pre-hematopoietic stem cell formation from endothelium |
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| Relations |
| BioProject |
PRJNA564578 |
| SRA |
SRP221064 |
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