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Series GSE137050 Query DataSets for GSE137050
Status Public on May 06, 2022
Title The comparative roadmaps of reprogramming and transformation unveiled that cellular plasticity is broadly controlled by Bcl11b and Atoh8
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Coordinated changes of cellular plasticity and cellular identity are critical for pluripotent reprogramming and oncogenic transformation. However, the sequences of cellular/molecular events that orchestrate these intermingled modifications have never been comparatively dissected. Here, we deconvoluted the cellular trajectories of reprogramming (via Oct4/Sox2/Klf4/c-Myc) and transformation (via Ras/c-Myc) at the single-cell resolution and revealed how the two processes intersect prior to bifurcate. This approach also led to identify the transcription factor (TF) Bcl11b as a broad-range regulator of cell fate changes, as well as a pertinent marker to capture early cellular intermediates that emerge simultaneously during reprogramming & transformation. Multi-omics characterization of these intermediates led to unveil a c-Myc/Atoh8/Sfrp1 regulatory axis that constrains rodent and human reprogramming but also cancer cell plasticity and neuron transdifferentiation. Mechanistically, we found that the TF Atoh8 restrains cellular plasticity, independently of cellular identity, by binding a specific enhancer network. This study provides insights into the partitioned control of cellular plasticity and identity for both regenerative and cancer biology.
Overall design Refer to individual Series
Citation(s) 36075976
Submission date Sep 06, 2019
Last update date Oct 04, 2022
Contact name Fabrice Lavial
Organization name CRCL
Street address 28 rue Laennec
City Lyon
ZIP/Postal code 69008
Country France
Platforms (4)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (59)
GSM4263584 MEF sh#Control rep1
GSM4263585 MEF sh#Control rep2
GSM4263586 MEF sh#Atoh8 rep1
This SuperSeries is composed of the following SubSeries:
GSE143592 Identification of genes upregulated and downregulated in mouse embryonic fibroblasts (MEFs) upon Atoh8 depletion
GSE143593 Identification of genes upregulated and downregulated in transformed cells (TCs) derived upon Atoh8 depletion
GSE181196 Bcl11b and Atoh8 binding on chromatin in MEFs
BioProject PRJNA564325

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Supplementary file Size Download File type/resource
GSE137050_RAW.tar 2.1 Gb (http)(custom) TAR (of BEDGRAPH, MTX, TSV, TXT, XLS)
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