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| Status |
Public on Jun 28, 2021 |
| Title |
RNA-seq of human myoblasts where AR isoform 2 was either knocked-down or upregulated. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study is to unravel the function of this AR isoform in the cell. In particular we are interested in understanding the function of AR isoform 2 on the biology of AR.
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| Overall design |
RNA-seq analysis was performed comparing the data set from human myoblasts transfected with plasmid expressing AR-2 (AR45) or mock sequence. Additionally RNA-seq analysis was also performed comparing data from human myoblasts treated with siRNA targeting AR-2 expression specifically or mock sequence. Conclusion: our study shows that AR-2 mainly alter genes containing an androgen responsive element in their promoter region.
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| Contributor(s) |
Dabney J, Rinaldi C |
| Citation(s) |
34417184 |
| |
| Submission date |
Sep 03, 2019 |
| Last update date |
Sep 29, 2021 |
| Contact name |
Carlo Rinaldi |
| E-mail(s) |
carlo.rinaldi@paediatrics.ox.ac.uk
|
| Organization name |
University of Oxford
|
| Department |
Department of Paediatrics
|
| Street address |
South Parks Road
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| City |
Oxford |
| ZIP/Postal code |
OX3 1QX |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA563704 |
| SRA |
SRP220194 |
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