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Series GSE136752 Query DataSets for GSE136752
Status Public on Jul 28, 2020
Title PHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Spermatogenesis is a complex process of sperm generation, including mitosis, meiosis, and spermiogenesis. During spermiogenesis, histones in post-meiotic spermatids are removed from chromatin and replaced by protamines. Although histone-to-protamine exchange is important for sperm nuclear condensation, the underlying regulatory mechanism is still poorly understood. Here, we identify PHD finger protein 7 (PHF7) as an E3 ubiquitin ligase for histone H3K14 in post-meiotic spermatids. Generation of Phf7-deficient mice and Phf7 C160A knockin mice with impaired E3 ubiquitin ligase activity reveals defects in histone-to-protamine exchange caused by dysregulation of histone removal factor Bromodomain, testis-specific (BRDT) in early condensing spermatids. Surprisingly, E3 ubiquitin ligase activity of PHF7 on histone ubiquitination leads to stabilization of BRDT by attenuating ubiquitination of BRDT. Collectively, our findings identify PHF7 as a critical factor for sperm chromatin condensation and contribute to mechanistic understanding of fundamental phenomenon of histone-to-protamine exchange and potential for drug development for the male reproduction system.
Overall design single cell RNA sequencing performed with testicular cells from Phf7 f/f and Phf7 tKO mice.
Contributor(s) Kim CR, Park S, Jung I, Baek SH
Citation(s) 32726616
Submission date Sep 03, 2019
Last update date Nov 03, 2020
Contact name Inkyung Jung
Organization name KAIST
Department Biological Sciences
Street address KAIST, 291 Daehak-ro, Yuseong-gu
City Daejeon
ZIP/Postal code 34141
Country South Korea
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM4331580 Phf7 f/f rep1
GSM4331581 Phf7 f/f rep2
GSM4331582 Phf7 tKO rep1
BioProject PRJNA563578
SRA SRP220113

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Supplementary file Size Download File type/resource
GSE136752_SGdev.barcodes.tsv.gz 155.7 Kb (ftp)(http) TSV
GSE136752_SGdev.features.tsv.gz 250.8 Kb (ftp)(http) TSV
GSE136752_SGdev.matrix.mtx.gz 183.0 Mb (ftp)(http) MTX
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