GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE136719 Query DataSets for GSE136719
Status Public on Oct 27, 2021
Title Single-cell transcription mapping of neural subtype development in human spinal cord
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The spinal cord possesses precise neural circuitry to transmit messages between the brain and body. Detailed transcriptomic profiling of the developing human spinal cord has not been reported. Here, we performed single cell RNA sequencing of developing human spinal cord cells and compared these data with similar mouse spinal cord RNA sequencing datasets. The differentiation tendency of proliferative neural progenitor cells changed from neuronal to glial cells at gestational week (GW) 8 and we identified a diverse set of excitatory, inhibitory and motor neuron cell types. Human ventral neuronal differentiation occurred earlier than GW7, while DI4/5 interneurons are born between GW7–11. We identified glial cell molecular diversity and revealed that ependymal cell specification occurs before birth. We also demonstrate differences between human and mouse spinal cord, including unique cell subtypes, gene expression, neurotransmitter receptors, and glial differentiation timing. Our results offer insight into human spinal cord development.
Overall design Here we performed both nucleus single-cell RNA sequencing and cellular single-cell RNA sequencing analyses of more than 880,000 cells isolated from the human spinal cord at gestation week 7 to 25.
Contributor(s) Zhang Q, Wu X, Fan Y, Xiao Z, Jiang P, Zhao Y, Yang Y, Han S, Chen B, Han J, Sun M, Zhao G, hu Y, Dai J
Citation(s) 34605607
Submission date Sep 02, 2019
Last update date Feb 15, 2022
Contact name Xianming Wu
Phone +86-15313377580
Organization name Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
Street address Unit 2, No.1 West Beichen Road
City Beijing
State/province Chaoyang District
ZIP/Postal code 100101
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (78)
GSM4080376 GW10C_01NC
GSM4080377 GW10C_02
GSM4080378 GW10C_02NC
BioProject PRJNA565733
SRA SRP221744

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE136719_RAW.tar 5.1 Gb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap