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Series GSE136695 Query DataSets for GSE136695
Status Public on Dec 12, 2019
Title Altered Biogenesis and MicroRNA Content of Hippocampal Exosomes Following Experimental Status Epilepticus
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Here, we characterised the effects of experimental status epilepticus on the expression of exosome biosynthesis components and analysed microRNA content in exosome-enriched fractions prepared from the mouse hippocampus. Status epilepticus induced by unilateral intra-amygdala kainic acid resulted in acute subfield-specific, bi-directional changes in transcripts associated with exosome biosynthesis including up-regulation of ESCRT–dependent and –independent pathways. Increased expression of exosome components including Alix were detectable in samples obtained two weeks after status epilepticus and changes occurred in both the ipsilateral and contralateral hippocampus. Small RNAseq analysis of exosome-enriched fractions prepared using two different techniques detected a rich diversity of conserved microRNAs and determined status epilepticus selectively alters microRNA contents, including upregulation of the glia-enriched miR-21a-3p. We also characterized editing sites of the exosome-enriched miRNAs and found six exosome-enriched miRNAs that were Adenosine-to-Inosine (ADAR) edited with the majority of the editing events predicted to occur within miRNA seed regions. However, the prevalence of these editing events was not altered by status epilepticus. These studies demonstrate status epilepticus alters the exosome pathway and its microRNA content, but not editing patterns.
Overall design Exosomes were extracted using two different methods (ultracentrifugation or using commercial extraction kit (ExoQuick) at two different time points (24 hrs and 2 weeks). Small RNA librarires were prepared from the exosomes and sequenced on Illumina Mi-Seq. Each group has 3 biological samples.
Contributor(s) Batool A, Hill TD, Nguyen NT, Langa E, Diviney M, Mooney C, Brennan GP, Connolly NC, Sanz-Rodriguez A, Cavanagh B, Henshall DC
Citation(s) 32009885
Submission date Aug 30, 2019
Last update date Mar 12, 2020
Contact name David Henshall
Organization name FutureNeuro centre, Royal College of Surgeons in Ireland
Street address 123 Saint Stephen Green
City Dublin 2
ZIP/Postal code D02 YN77
Country Ireland
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (24)
GSM4055574 UC control 24h rep1
GSM4055575 UC KA 24h rep1
GSM4055576 UC control 2w rep1
BioProject PRJNA563073
SRA SRP219953

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Supplementary file Size Download File type/resource
GSE136695_RAW.tar 720.0 Kb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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