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Series GSE136679 Query DataSets for GSE136679
Status Public on Sep 14, 2020
Title Functional Compensation Precedes Recovery of Tissue Mass Following Acute Liver Injury
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We identify a new phase of functional compensation following acute liver injury that occurs prior to cellular proliferation. By coupling single-cell RNA-seq with in situ transcriptional analyses in two independent murine liver injury models, we discover adaptive reprogramming to ensure expression of both injury response and core liver function genes dependent on macrophage-derived WNT/b-catenin signaling. Interestingly, transcriptional compensation is most prominent in non-proliferating cells, clearly delineating two separate phases of liver recovery. Overall, our work describes a new mechanism by which the liver maintains essential physiological functions prior to cellular reconstitution and characterizes macrophage-derived WNT signals required for this compensation.
Overall design Examination by single-cell RNA-Seq of hepatocyte response to acute liver injury at multiple time points in an acetaminophen toxicity and a partial hepatectomy injury model. Untreated controls are also included.
Contributor(s) Kolb K, Shalek A
Citation(s) 33214549
Submission date Aug 30, 2019
Last update date Dec 01, 2020
Contact name Alex K Shalek
Phone 6173245670
Organization name MIT
Department Chemistry
Lab Shalek Lab
Street address 77 Massachusetts Avenue
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (19)
GSM4055127 UTM1
GSM4055128 UTM5
GSM4055129 UTM6
BioProject PRJNA563047
SRA SRP219811

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE136679_ALI_allcell_400gene.txt.gz 32.7 Mb (ftp)(http) TXT
GSE136679_ALI_filterHQhep.txt.gz 202.0 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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