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| Status |
Public on Sep 04, 2020 |
| Title |
Time-resolved RNA atlas of early human T cell activation (mRNA) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
MicroRNAs are central regulators of the T cell function. We explored RNA expression profiles over the initial 24 h of human CD4+ T cell activation. We found high similarity in time-resolved miRNA expression courses comparing independent activations and different donors. The detected miRNA expression patterns could be grouped into six classes only, each with a defined time course. MiR-155-5p known for its role in T cell immunity showed the most prevalent expression changes, quantified with an hourly increase of about 60 molecules/cell. As demonstrated for miRNA-155-5p, the analysis of time-resolved miRNA and mRNA expression data allowed to increase the validation rate of predicted miRNA targets to close to 90 %. Combining our time-resolved expression analysis with an absolute quantification of miRNA expression changes, gives new insights into miRNA regulatory networks and indicates the functional dominance of specific miRNAs within the early T cell activation.
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| Overall design |
Early human T cell activation
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| Contributor(s) |
Diener C, Hart M |
| Citation(s) |
32990751 |
| Submission date |
Aug 29, 2019 |
| Last update date |
Nov 08, 2020 |
| Contact name |
Tim Kehl |
| E-mail(s) |
tkehl@bioinf.uni-sb.de
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| Phone |
+49-681-302-68613
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| Organization name |
Saarland University
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| Department |
Center for Bioinformatics
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| Street address |
Campus E2.1, P.O. box 151150
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| City |
Saarbrücken |
| ZIP/Postal code |
66041 |
| Country |
Germany |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (79)
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| GSM4053175 |
Donor 1, Timepoint 4, Replicate A |
| GSM4053176 |
Donor 1, Timepoint 6, Replicate A |
| GSM4053177 |
Donor 1, Timepoint 8, Replicate A |
| GSM4053178 |
Donor 1, Timepoint 10, Replicate A |
| GSM4053179 |
Donor 1, Timepoint 12, Replicate A |
| GSM4053180 |
Donor 1, Timepoint 14, Replicate A |
| GSM4053181 |
Donor 1, Timepoint 16, Replicate A |
| GSM4053182 |
Donor 1, Timepoint 18, Replicate A |
| GSM4053183 |
Donor 1, Timepoint 20, Replicate A |
| GSM4053184 |
Donor 1, Timepoint 22, Replicate A |
| GSM4053185 |
Donor 1, Timepoint 24, Replicate A |
| GSM4053186 |
Donor 1, Timepoint 0, Replicate B |
| GSM4053187 |
Donor 1, Timepoint 2, Replicate B |
| GSM4053188 |
Donor 1, Timepoint 4, Replicate B |
| GSM4053189 |
Donor 1, Timepoint 6, Replicate B |
| GSM4053190 |
Donor 1, Timepoint 8, Replicate B |
| GSM4053191 |
Donor 1, Timepoint 10, Replicate B |
| GSM4053192 |
Donor 1, Timepoint 12, Replicate B |
| GSM4053193 |
Donor 1, Timepoint 14, Replicate B |
| GSM4053194 |
Donor 1, Timepoint 16, Replicate B |
| GSM4053195 |
Donor 1, Timepoint 18, Replicate B |
| GSM4053196 |
Donor 1, Timepoint 20, Replicate B |
| GSM4053197 |
Donor 1, Timepoint 22, Replicate B |
| GSM4053198 |
Donor 1, Timepoint 24, Replicate B |
| GSM4053199 |
Donor 1, Timepoint 0, Replicate C |
| GSM4053200 |
Donor 1, Timepoint 2, Replicate C |
| GSM4053201 |
Donor 1, Timepoint 4, Replicate C |
| GSM4053202 |
Donor 1, Timepoint 6, Replicate C |
| GSM4053203 |
Donor 1, Timepoint 8, Replicate C |
| GSM4053204 |
Donor 1, Timepoint 10, Replicate C |
| GSM4053205 |
Donor 1, Timepoint 12, Replicate C |
| GSM4053206 |
Donor 1, Timepoint 14, Replicate C |
| GSM4053207 |
Donor 1, Timepoint 16, Replicate C |
| GSM4053208 |
Donor 1, Timepoint 18, Replicate C |
| GSM4053209 |
Donor 1, Timepoint 20, Replicate C |
| GSM4053210 |
Donor 1, Timepoint 22, Replicate C |
| GSM4053211 |
Donor 1, Timepoint 24, Replicate C |
| GSM4053212 |
Donor 2, Timepoint 0, Replicate A |
| GSM4053213 |
Donor 2, Timepoint 2, Replicate A |
| GSM4053214 |
Donor 2, Timepoint 4, Replicate A |
| GSM4053215 |
Donor 2, Timepoint 6, Replicate A |
| GSM4053216 |
Donor 2, Timepoint 8, Replicate A |
| GSM4053217 |
Donor 2, Timepoint 10, Replicate A |
| GSM4053218 |
Donor 2, Timepoint 12, Replicate A |
| GSM4053219 |
Donor 2, Timepoint 14, Replicate A |
| GSM4053220 |
Donor 2, Timepoint 16, Replicate A |
| GSM4053221 |
Donor 2, Timepoint 18, Replicate A |
| GSM4053222 |
Donor 2, Timepoint 20, Replicate A |
| GSM4053223 |
Donor 2, Timepoint 22, Replicate A |
| GSM4053224 |
Donor 2, Timepoint 24, Replicate A |
| GSM4053225 |
Donor 2, Timepoint 0, Replicate B |
| GSM4053226 |
Donor 2, Timepoint 2, Replicate B |
| GSM4053227 |
Donor 2, Timepoint 4, Replicate B |
| GSM4053228 |
Donor 2, Timepoint 6, Replicate B |
| GSM4053229 |
Donor 2, Timepoint 8, Replicate B |
| GSM4053230 |
Donor 2, Timepoint 10, Replicate B |
| GSM4053231 |
Donor 2, Timepoint 12, Replicate B |
| GSM4053232 |
Donor 2, Timepoint 14, Replicate B |
| GSM4053233 |
Donor 2, Timepoint 16, Replicate B |
| GSM4053234 |
Donor 2, Timepoint 18, Replicate B |
| GSM4053235 |
Donor 2, Timepoint 20, Replicate B |
| GSM4053236 |
Donor 2, Timepoint 22, Replicate B |
| GSM4053237 |
Donor 2, Timepoint 24, Replicate B |
| GSM4053238 |
Donor 2, Timepoint 0, Replicate C |
| GSM4053239 |
Donor 2, Timepoint 2, Replicate C |
| GSM4053240 |
Donor 2, Timepoint 4, Replicate C |
| GSM4053241 |
Donor 2, Timepoint 6, Replicate C |
| GSM4053242 |
Donor 2, Timepoint 8, Replicate C |
| GSM4053243 |
Donor 2, Timepoint 10, Replicate C |
| GSM4053244 |
Donor 2, Timepoint 12, Replicate C |
| GSM4053245 |
Donor 2, Timepoint 14, Replicate C |
| GSM4053246 |
Donor 2, Timepoint 16, Replicate C |
| GSM4053247 |
Donor 2, Timepoint 18, Replicate C |
| GSM4053248 |
Donor 2, Timepoint 20, Replicate C |
| GSM4053249 |
Donor 2, Timepoint 22, Replicate C |
| GSM4053250 |
Donor 2, Timepoint 24, Replicate C |
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| Relations |
| BioProject |
PRJNA562891 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE136625_RAW.tar |
978.1 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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