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Series GSE136625 Query DataSets for GSE136625
Status Public on Sep 04, 2020
Title Time-resolved RNA atlas of early human T cell activation (mRNA)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary MicroRNAs are central regulators of the T cell function. We explored RNA expression profiles over the initial 24 h of human CD4+ T cell activation. We found high similarity in time-resolved miRNA expression courses comparing independent activations and different donors. The detected miRNA expression patterns could be grouped into six classes only, each with a defined time course. MiR-155-5p known for its role in T cell immunity showed the most prevalent expression changes, quantified with an hourly increase of about 60 molecules/cell. As demonstrated for miRNA-155-5p, the analysis of time-resolved miRNA and mRNA expression data allowed to increase the validation rate of predicted miRNA targets to close to 90 %. Combining our time-resolved expression analysis with an absolute quantification of miRNA expression changes, gives new insights into miRNA regulatory networks and indicates the functional dominance of specific miRNAs within the early T cell activation.
 
Overall design Early human T cell activation
 
Contributor(s) Diener C, Hart M
Citation(s) 32990751
Submission date Aug 29, 2019
Last update date Nov 08, 2020
Contact name Tim Kehl
E-mail(s) tkehl@bioinf.uni-sb.de
Phone +49-681-302-68613
Organization name Saarland University
Department Center for Bioinformatics
Street address Campus E2.1, P.O. box 151150
City Saarbr├╝cken
ZIP/Postal code 66041
Country Germany
 
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (79)
GSM4053172 Donor 1, Timepoint 0, Replicate A
GSM4053173 Donor 1, Timepoint 0, Replicate A2
GSM4053174 Donor 1, Timepoint 2, Replicate A
Relations
BioProject PRJNA562891

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE136625_RAW.tar 978.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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