|
| Status |
Public on Feb 21, 2021 |
| Title |
SRPK3 regulates alternative pre-mRNA splicing required for B lymphocyte development and humoral responsiveness |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Alternative splicing (AS) of pre-mRNA is a critical component of transcriptional regulation that diversifies the cellular proteome. The Serine-Arginine Protein Kinases (SRPK) initiate early events in AS. Using conditional knockout mice (cKO), we demonstrated the importance of the X-linked Srpk3 gene in B lymphocyte development and in response to immunization in vivo. Significantly decreased numbers of immature and mature B cells were observed in Srpk3-cKO mouse bone marrow relative to wild-type (WT). Immunization of Srpk3-cKO mice with the T lymphocyte-independent type 2 antigen 4-hydroxy-3-nitrophenylacetyl-Ficoll (NP-Ficoll) elicited greatly reduced amounts of NP-specific IgG3. Srpk3 deletion resulted in hundreds of differentially spliced mRNAs in B cells, including mRNAs encoding proteins associated with signaling pathways and mitochondrial function. We conclude that Srpk3 is an immunomodulatory kinase that controls humoral immunity via its regulation of pre-mRNA splicing, antibody production, and metabolism in B cells.
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| |
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| Overall design |
Profiling of RNA metabolism in immune cells from wildtype and SRPK3 knockout mice
|
| Web link |
https://doi.org/10.1101/759829
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| |
|
| Contributor(s) |
Taliaferro M, Hagman J, Arends T |
| Citation missing |
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| Submission date |
Aug 29, 2019 |
| Last update date |
Feb 21, 2021 |
| Contact name |
Matthew Taliaferro |
| Organization name |
University of Colorado Anschutz Medical Campus
|
| Department |
Biology
|
| Street address |
12801 E 17th Ave, RC1 South Room 10403D
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA562889 |
| SRA |
SRP219639 |
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