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Series GSE136622 Query DataSets for GSE136622
Status Public on Feb 21, 2021
Title SRPK3 regulates alternative pre-mRNA splicing required for B lymphocyte development and humoral responsiveness
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Alternative splicing (AS) of pre-mRNA is a critical component of transcriptional regulation that diversifies the cellular proteome. The Serine-Arginine Protein Kinases (SRPK) initiate early events in AS. Using conditional knockout mice (cKO), we demonstrated the importance of the X-linked Srpk3 gene in B lymphocyte development and in response to immunization in vivo. Significantly decreased numbers of immature and mature B cells were observed in Srpk3-cKO mouse bone marrow relative to wild-type (WT). Immunization of Srpk3-cKO mice with the T lymphocyte-independent type 2 antigen 4-hydroxy-3-nitrophenylacetyl-Ficoll (NP-Ficoll) elicited greatly reduced amounts of NP-specific IgG3. Srpk3 deletion resulted in hundreds of differentially spliced mRNAs in B cells, including mRNAs encoding proteins associated with signaling pathways and mitochondrial function. We conclude that Srpk3 is an immunomodulatory kinase that controls humoral immunity via its regulation of pre-mRNA splicing, antibody production, and metabolism in B cells.
Overall design Profiling of RNA metabolism in immune cells from wildtype and SRPK3 knockout mice
Web link
Contributor(s) Taliaferro M, Hagman J, Arends T
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Submission date Aug 29, 2019
Last update date Feb 21, 2021
Contact name Matthew Taliaferro
Organization name University of Colorado Anschutz Medical Campus
Department Biology
Street address 12801 E 17th Ave, RC1 South Room 10403D
City Aurora
State/province CO
ZIP/Postal code 80045
Country USA
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (36)
GSM4053118 WT_unstim_rep1
GSM4053119 WT_unstim_rep2
GSM4053120 WT_unstim_rep3
BioProject PRJNA562889
SRA SRP219639

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Supplementary file Size Download File type/resource
GSE136622_RAW.tar 61.3 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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