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Series GSE136417 Query DataSets for GSE136417
Status Public on Jul 31, 2020
Title Maternal exposure to a mitochondrial toxicant results in life-long alterations in DNA methylation and gene expression in the offspring
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary We exposed dams to rotenone starting 2 weeks prior to conception all the way to the end of lactation to determine its effects on the DNA methylation landscape of the offspring. We find that this treatment not only affects the DNA methylation status in one locus in the skin (both doses were studied here) but the entire DNA methylome of the liver is also changed (only lower dose was analyzed). The effects last throughout the lifespan of the animals and are accompanied by changes in the transcriptome.
Overall design Whole genome bisulfite sequencing (WGBS) and RNA-seq were performed using livers from animals in the control group or exposed perinatally to rotenone at PND22, 6, 12 and 18 months of age. WGBS and RNA-seq Samples with the same title and replicate number are derived from the same liver sample.
Contributor(s) Lozoya OA, Xu F, Woychik RP, Santos JH
Citation(s) 32937126
Submission date Aug 27, 2019
Last update date Oct 20, 2020
Contact name Janine Santos
Organization name NIEHS
Department GISBL
Street address 111 TW Alexander drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
Platforms (2)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (66)
GSM4048705 D22-0ppm-female-rep1.RNAseq
GSM4048706 D22-0ppm-female-rep2.RNAseq
GSM4048707 D22-0ppm-female-rep3.RNAseq
BioProject PRJNA562525
SRA SRP219391

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE136417_RAW.tar 8.9 Gb (http)(custom) TAR (of BIGWIG, TXT)
GSE136417_RNAseq_RPKM_matrix.txt.gz 2.3 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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