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Series GSE136376 Query DataSets for GSE136376
Status Public on Jul 07, 2020
Title scRNA-seq of T cell Independent B cell responses
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To fine map the continuum of molecular changes that occur as B cells differentiate to antibody secreting plasma cells in response to the T cell independent antigens LPS and NP-Ficoll we performed scRNA-seq. WT or IRF4-deficient B cells were CTV labeled and adoptively transferred into congenically disparate uMT hosts. One day later, the mice were innoculated with LPS and all transferred cells isolated at 72 hours and profiled on the 10x Genomimcs 5' Library Kit. In addition, we adoptively transferred WT B cells into congenically disparate WT hosts and innoculated the animals with either LPS or NP-Ficoll, and 72 hours later performed scRNA-seq on all responding B cells.
Overall design scRNA-seq on two independent biological replicates of wild-type or IRF4-deficient B cells responding to LPS in uMT mice and wild-type B cells responding to LPS or NP-Ficoll in wild-type hosts.
Contributor(s) Scharer CD, Boss JM
Citation(s) 32778653
NIH grant(s)
Grant ID Grant title Affiliation Name
P01 AI125180 Plasma Cells in Health and Disease EMORY UNIVERSITY Ignacio E. Sanz
R01 AI123733 Epigenetic Regulation of Plasma Cells Differentiation EMORY UNIVERSITY JEREMY M. BOSS
F31 AI138391 Epigenetic Regulation of Memory B Cell Differentiation and Reactivation EMORY UNIVERSITY Madeline Joan Price
Submission date Aug 26, 2019
Last update date Aug 13, 2020
Contact name Chris Scharer
Organization name Emory University
Department Microbiology and Immunology
Lab Chris Scharer
Street address 1510 Clifton Rd, Suite 3086A
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM4047602 WT_1
GSM4047603 WT_2
GSM4047604 IRF4null_1
BioProject PRJNA562315
SRA SRP219696

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Supplementary file Size Download File type/resource
GSE136376_IRF4null_UMI_table_filtered.csv.gz 19.7 Mb (ftp)(http) CSV
GSE136376_WTLPS_UMI_matrix.tsv.gz 13.8 Mb (ftp)(http) TSV
GSE136376_WTNPF_UMI_matrix.tsv.gz 9.3 Mb (ftp)(http) TSV
GSE136376_WT_UMI_table_filtered.csv.gz 30.3 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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