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Series GSE136245 Query DataSets for GSE136245
Status Public on Oct 31, 2019
Title Active and repressed chromatin domains exhibit distinct nucleosome segregation during DNA replication
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Chromatin domains and their associated structures must be faithfully inherited through cellular division to maintain cellular identity. Yet, accessing the localized strategies preserving chromatin domain inheritance, specifically the transfer of parental, pre-existing nucleosomes with their associated post-translational modifications (PTMs) during DNA replication is challenging in living cells. We devised an inducible, proximity-dependent labeling system to irreversibly mark replication-dependent H3.1 and H3.2 histone-containing nucleosomes at single desired loci in mouse embryonic stem cells such that their fate after DNA replication could be followed. Strikingly, repressed chromatin domains are preserved through the local re-deposition of parental nucleosomes. In contrast, nucleosomes decorating active chromatin domains do not exhibit such preservation. Notably, altering cell fate leads to an adjustment in the positional inheritance of parental nucleosomes that reflects the corresponding changes in chromatin structure. These findings point to important mechanisms that contribute to parental nucleosome segregation to preserve cellular identity.
Overall design A method that permanently labels histones at chosen loci revealed that nucleosomes from repressed (but not active) chromatin domains are re-deposited locally after DNA replication.
Contributor(s) Escobar TM, Oksuz O, Saldaña-Meyer R, Descostes N, Bonasio R, Reinberg D
Citation(s) 31675501
Submission date Aug 23, 2019
Last update date Nov 08, 2019
Contact name Danny Reinberg
Organization name NYU school of Medicine
Department Biochemistry
Lab Reinberg
Street address 522 first avenue
City new york
State/province new york
ZIP/Postal code 10016
Country USA
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (27)
GSM4043203 EBF1_Biotin_No_Dox
GSM4043204 EBF1_Biotin_Plus_Dox
GSM4043205 EBF1_input
BioProject PRJNA561753
SRA SRP219192

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Supplementary file Size Download File type/resource
GSE136245_RAW.tar 3.3 Gb (http)(custom) TAR (of BW, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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