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| Status |
Public on Oct 31, 2019 |
| Title |
Active and repressed chromatin domains exhibit distinct nucleosome segregation during DNA replication |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Chromatin domains and their associated structures must be faithfully inherited through cellular division to maintain cellular identity. Yet, accessing the localized strategies preserving chromatin domain inheritance, specifically the transfer of parental, pre-existing nucleosomes with their associated post-translational modifications (PTMs) during DNA replication is challenging in living cells. We devised an inducible, proximity-dependent labeling system to irreversibly mark replication-dependent H3.1 and H3.2 histone-containing nucleosomes at single desired loci in mouse embryonic stem cells such that their fate after DNA replication could be followed. Strikingly, repressed chromatin domains are preserved through the local re-deposition of parental nucleosomes. In contrast, nucleosomes decorating active chromatin domains do not exhibit such preservation. Notably, altering cell fate leads to an adjustment in the positional inheritance of parental nucleosomes that reflects the corresponding changes in chromatin structure. These findings point to important mechanisms that contribute to parental nucleosome segregation to preserve cellular identity.
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| Overall design |
A method that permanently labels histones at chosen loci revealed that nucleosomes from repressed (but not active) chromatin domains are re-deposited locally after DNA replication.
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| Contributor(s) |
Escobar TM, Oksuz O, Saldaña-Meyer R, Descostes N, Bonasio R, Reinberg D |
| Citation(s) |
31675501 |
| |
| Submission date |
Aug 23, 2019 |
| Last update date |
Nov 08, 2019 |
| Contact name |
Danny Reinberg |
| E-mail(s) |
danny.reinberg@nyulangone.org
|
| Organization name |
NYU school of Medicine
|
| Department |
Biochemistry
|
| Lab |
Reinberg
|
| Street address |
522 first avenue
|
| City |
new york |
| State/province |
new york |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (27)
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| Relations |
| BioProject |
PRJNA561753 |
| SRA |
SRP219192 |
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