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Series GSE136184 Query DataSets for GSE136184
Status Public on Jun 28, 2022
Title Single Cell Analysis of CD8+ T-cells with Aging
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary CD8+ T cells play essential roles in combatting infection and cancers. The major subsets of CD8+ T cells such as naïve, effector, and memory carry out distinct functions. Although the defined subsets are known to be heterogeneous, the precise composition of these subsets and their functional alterations have not been thoroughly determined. Here, we used single cell RNA sequencing (scRNAseq) and multicolor immunophenotyping to analyze the composition of CD8+ T cells. From scRNAseq analysis of isolated CD8+ T cells from 16 healthy donors, we analyzed 80,775 cells and identified a total of 13 subpopulations of CD8+ T cells including naïve, central and effector memory cells and terminally differentiated effector memory cells (EMRA). In parallel, our multi-color (16 antibody-panel) immunophenotyping analysis by flow cytometry of CD8+ T cells from 165 donors also divided CD8+ T cells into 11 major subpopulations. The majority of the subpopulations identified by these two methods were their age-related changes in percentage. Together, our findings reveal previously unidentified subpopulations of CD8+ T cells, their distinct transcriptional profiles and changes with age.
Overall design single cell gene expression of CD8+ T-cells from 16 individuals spanning from the newborn to the elderly
Contributor(s) Lu J, Ahmad R, Nguyen T
Citation(s) 36050300
Submission date Aug 22, 2019
Last update date Dec 07, 2022
Contact name Nan-Ping Weng
Organization name NIA
Department LMBI
Street address 251 Bayview Blvd
City Baltimore
ZIP/Postal code 21224
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (16)
GSM4042196 sc_Donor_1_GEX
GSM4042197 sc_Donor_2_GEX
GSM4042198 sc_Donor_3_GEX
BioProject PRJNA561519
SRA SRP219091

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Supplementary file Size Download File type/resource
GSE136184_rsc_Gene_Expression_Aging_UMI_Counts2.Rds.gz 262.9 Mb (ftp)(http) RDS
GSE136184_seu_obj2.Rds.gz 5.6 Gb (ftp)(http) RDS
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Raw data are available in SRA
Processed data are available on Series record

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