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Series GSE135837 Query DataSets for GSE135837
Status Public on Mar 31, 2020
Title Estrogen receptor β regulates skeletal muscle mass and muscle stem cell proliferation in young female mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The female sex hormone estrogens plays a critical role in maintaining muscle mass and muscle stem cell (MuSCs) functions. However, it is still unclear about downstream pathways of estrogens including its receptors that are expressed in both skeletal muscle tissue and MuSCs. To study the specific role of estrogen receptor β (ERβ), one of two main types of estrogen receptors, in skeletal muscle and MuSCs, we generated muscle-specific ERβ-knockout (mKO) mice and muscle stem cell-specific ERβ-knockout (scKO) mice. Here, we show that muscle-specific ERβ-deficient induced decreased muscle strength and fast-type muscle mass in young female mice. Furthermore, muscle stem cell-specific ERβ-deficient young female mice but not male exhibited impaired muscle regeneration ability after acute muscle injury, accompanied by a decreased proliferation rate of muscle stem cells. RNA sequencing analysis showed that the loss of ERβ in muscle stem cells changes the expression of cell cycle associated genes and niche component factors including laminin and collagen. Thus, our characterization of mKO and scKO mice indicate that the estrogen-ERβ pathway is a sex-specific regulatory mechanism that controls both skeletal muscle mass and the proliferation of muscle stem cell in females and could be of importance in a therapeutic context.
Overall design mRNA profiles of primary satellite cells obtained from Control and ERβ cKO mice were generated by deep sequencing, in triplicate, using Illumina Miseq.
Contributor(s) Ono Y, Fujita R
Citation(s) 32822588
Submission date Aug 14, 2019
Last update date Oct 02, 2020
Contact name Ryo Fujita
Organization name University of Tsukuba
Department Transborder Medical Research Center
Lab Division of Regenerative Medicine
Street address 1-1-1
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8577
Country Japan
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (4)
GSM4034997 Control rep1
GSM4034998 Control rep2
GSM4034999 ERβ scKO rep1
BioProject PRJNA560238
SRA SRP218357

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Supplementary file Size Download File type/resource
GSE135837_ERbeta_KO_RNA-seq_n_2.xlsx 3.1 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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