|
| Status |
Public on May 19, 2020 |
| Title |
NelfA (Whsc2) promoter occupancy is required for active gene transcription during cardiac hypertrophy |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We examineD the genomic oppucpany and role of negative elongation factor A (NelfA) aka, Wolf-Hirschhorn syndrome candidate 2 (Whsc2), a critical component of the negative elongation complex in hearts undergoing pressure-overload induced cardiac hypertrophy. Alignment of high resolution genome wide occupancy data of NelfA, RNA Pol II, TFIIB and H3k9ac (datasets for pol II, TFIIB and H3kac submitted before) from control and hypertrophied hearts reveal that NelfA associates with all active gene promoters. High NelfA occupancy is seen at promoters of essential and cardiac-enriched genes, expressed under both quiescent and hypertrophic conditions. Conversely, de novo NelfA recruitment is observed at inducible gene promoters with pressure overload, accompanied by significant increase in expression of latter genes with hypertrophy.
|
| |
|
| Overall design |
Hearts were extracted from C57/Blk mice subjected to sham ot TAC operations. Hearts were sent to Active Motif, Inc for NelfA_ChIP-Seq
|
| |
|
| Contributor(s) |
Sayed D |
| Citation(s) |
32278832 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HL128799 |
NOVEL POSTTRANSCRIPTIONAL REGULATORS OF STRESS HORMONE GLUCOCORTICOID RECEPTOR?DEPENDENT GENE TRANSCRIPTION DURING CARDIAC HYPERTROPHY AND FAILURE |
RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY--RBHS-NEW JERSEY MEDICAL SCHOOL |
Danish Sayed |
|
| |
| Submission date |
Aug 14, 2019 |
| Last update date |
May 19, 2020 |
| Contact name |
Danish Sayed |
| E-mail(s) |
sayeddh@njms.rutgers.edu
|
| Phone |
(973) 972-5243
|
| Organization name |
Rutgers-NJMS
|
| Department |
CBMM
|
| Lab |
MSB G 626
|
| Street address |
185 south orange avenue
|
| City |
Newark |
| State/province |
NJ |
| ZIP/Postal code |
07103 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (3) |
|
| Relations |
| BioProject |
PRJNA560222 |
| SRA |
SRP218338 |
