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Series GSE135815 Query DataSets for GSE135815
Status Public on Mar 13, 2020
Title Evolutionary conserved NSL complex/BRD4 axis controls transcription activation via histone acetylation
Organisms Drosophila melanogaster; Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Cells rely on a diverse repertoire of genes for maintaining homeostasis, but the transcriptional networks underlying their expression remain poorly understood. The MOF acetyltransferase-containing Non-Specific Lethal (NSL) complex is a broad transcription regulator. It is essential in Drosophila and haploinsufficiency of the human KANSL1 subunit results in the Koolen-de Vries syndrome. Here, we perform a genome-wide RNAi screen and identify the BET protein BRD4 as evolutionary conserved co-factor of the NSL complex. Using Drosophila and mouse embryonic stem cells, we characterise a recruitment hierarchy, where NSL-deposited histone acetylation induces BRD4 recruitment for transcription of constitutively active genes. Transcriptome analyses in Koolen-de Vries patient-derived fibroblasts reveals perturbations with a cellular homeostasis signature that are evoked by the NSL complex/BRD4-axis. We propose that BRD4 represents a conserved bridge between the NSL complex and transcription activation and provide a new perspective in the understanding of their functions in healthy and diseased states.
 
Overall design This SuperSeries is composed of the SubSeries listed below.
 
Citation(s) 32382029, 37399316
Submission date Aug 14, 2019
Last update date Sep 11, 2023
Contact name Asifa Akhtar
E-mail(s) akhtarlab_data@ie-freiburg.mpg.de
Organization name Max Planck Institute of Immunobiology and Epigenetics
Department Chromatin Regulation
Lab Akhtar Lab
Street address Stuebeweg 51
City Freiburg
ZIP/Postal code 79108
Country Germany
 
Platforms (4)
GPL17275 Illumina HiSeq 2500 (Drosophila melanogaster)
GPL19132 Illumina NextSeq 500 (Drosophila melanogaster)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (84)
GSM4029602 Dm_S2cell_Biotin_dBRD4-S_ChIPseq_WT_untagged_control_rep1_Input
GSM4029603 Dm_S2cell_Biotin_dBRD4-S_ChIPseq_GST_RNAi_rep1_Input
GSM4029604 Dm_S2cell_Biotin_dBRD4-S_ChIPseq_NSL1_RNAi_rep1_Input
This SuperSeries is composed of the following SubSeries:
GSE135771 Evolutionary conserved NSL complex/BRD4 axis controls transcription activation via histone acetylation
GSE135772 Evolutionary conserved NSL complex/BRD4 axis controls transcription activation via histone acetylation
GSE135773 Evolutionary conserved NSL complex/BRD4 axis controls transcription activation via histone acetylation
Relations
BioProject PRJNA560185

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE135815_RAW.tar 3.1 Gb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
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