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Series GSE135783 Query DataSets for GSE135783
Status Public on Aug 13, 2022
Title The lncRNA RUNX1-IT1 promotes pancreatic cancer progression by inducing C-FOS expression
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The functional analysis of RUNX1-IT1 in tumors is relatively lacking, and its role and mechanism in pancreatic have not been reported. RUNX1-IT1 is transcribed from the intron of the RUNX1 gene, which plays an important role in solid tumors, and its abnormal expression is closely associated with cancer progression. However, due to the lack of systematic research, the regulatory mechanism and important downstream targets of RUNX1-IT1 and RUNX1 in PC are unclear. To investigate the downstream pathway of RUNX1-IT1 and RUNX1, we performed RNA-seq using two PANC-1 cell groups: RUNX1-IT1 knockdown and control, and RUNX1 knockdown and control.
Overall design PANC-1 cells were transfected with RUNX1-IT1 Smart Silencer and control Smart Silencer, and sh-RUNX1 and control lentiviral vectors. Total RNA was extracted from the samples using the TRIzol method. The total RNA extracted from the samples was tested by a NanoDrop 2000 and denaturing gel electrophoresis. RNA Sequencing Analysis were performed after the sample quality control.
Contributor(s) Wang H, Liu S, Zhang J
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Submission date Aug 13, 2019
Last update date Aug 13, 2022
Contact name Junfeng Zhang
Organization name First Affiliated Hospital of Army Medical University
Street address Gaotan Yanzheng Street, Shapingba District
City Chongqing
ZIP/Postal code 400038
Country China
Platforms (2)
GPL20795 HiSeq X Ten (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (11)
GSM4029985 sh-NC1(nc1)
GSM4029986 sh-NC2 (nc2)
GSM4029987 sh-NC3 (nc3)
BioProject PRJNA560049
SRA SRP218237

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Supplementary file Size Download File type/resource
GSE135783_RUNX1-IT1_gene_expression.txt.gz 3.1 Mb (ftp)(http) TXT
GSE135783_RUNX1_gene_expression.txt.gz 2.6 Mb (ftp)(http) TXT
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