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Series GSE135773 Query DataSets for GSE135773
Status Public on Mar 13, 2020
Title Evolutionary conserved NSL complex/BRD4 axis controls transcription activation via histone acetylation
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Cells rely on a diverse repertoire of genes for maintaining homeostasis, but the transcriptional networks underlying their expression remain poorly understood. The MOF acetyltransferase-containing Non-Specific Lethal (NSL) complex is a broad transcription regulator. It is essential in Drosophila and haploinsufficiency of the human KANSL1 subunit results in the Koolen-de Vries syndrome. Here, we perform a genome-wide RNAi screen and identify the BET protein BRD4 as evolutionary conserved co-factor of the NSL complex. Using Drosophila and mouse embryonic stem cells, we characterise a recruitment hierarchy, where NSL-deposited histone acetylation induces BRD4 recruitment for transcription of constitutively active genes. Transcriptome analyses in Koolen-de Vries patient-derived fibroblasts reveals perturbations with a cellular homeostasis signature that are evoked by the NSL complex/BRD4-axis. We propose that BRD4 represents a conserved bridge between the NSL complex and transcription activation and provide a new perspective in the understanding of their functions in healthy and diseased states.
Overall design Compartive expression analysis of Koolen-de-Vries patient and healthy control fibroblast cell lines
Contributor(s) Gaub A, Akhtar A
Citation(s) 32382029, 37399316
Submission date Aug 13, 2019
Last update date Sep 11, 2023
Contact name Asifa Akhtar
Organization name Max Planck Institute of Immunobiology and Epigenetics
Department Chromatin Regulation
Lab Akhtar Lab
Street address Stuebeweg 51
City Freiburg
ZIP/Postal code 79108
Country Germany
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (7)
GSM4029679 human_fibroblast_PolyA_RNAseq_KdV_patient1
GSM4029680 human_fibroblast_PolyA_RNAseq_KdV_patient2
GSM4029681 human_fibroblast_PolyA_RNAseq_KdV_patient3
This SubSeries is part of SuperSeries:
GSE135815 Evolutionary conserved NSL complex/BRD4 axis controls transcription activation via histone acetylation
BioProject PRJNA560038
SRA SRP218226

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Supplementary file Size Download File type/resource
GSE135773_human_fibroblast_RNA-seq_KdV_counts.tsv.gz 491.7 Kb (ftp)(http) TSV
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