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Series GSE135619 Query DataSets for GSE135619
Status Public on Aug 10, 2019
Title Robust hepatitis E virus infection and transcriptional response in human hepatocytes
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary In this study, we established a protocol based on the HEV genotype 3 p6 (Kernow C-1) and the human hepatoma cell lines HepG2 and HepG2/C3A with different media conditions to produce HEV cell culture-derived particles (HEVcc) with viral titers between 10e5 to 10e6 FFU/mL. Viral titers could be further enhanced by an HEV variant harboring a mutation in the RNA-dependent RNA polymerase. These HEVcc particles were infectious in primary human hepatocytes. RNA sequencing studies of HEV infected primary human hepatocytes demonstrated a temporally structured transcriptional defense response. In conclusion, this robust cell culture model of HEV infection provides a powerful tool for studying viral-host interactions that should facilitate the discovery of antiviral drugs and vaccines for this important zoonotic pathogen.
Overall design Primary human hepatocytes (PHH) were inoculated with cell culture-derived HEV particles and changes in transcriptional profiles were assessed using RNAseq.
Contributor(s) Todt D, Friesland M, Steinmann E
Citation(s) 31896581
Submission date Aug 09, 2019
Last update date Jan 06, 2020
Contact name Daniel Todt
Phone +492343222463
Organization name Ruhr University Bochum
Department Molecular & Medical Virology
Street address Universitätsstr. 150
City Bochum
ZIP/Postal code 44801
Country Germany
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (24)
GSM4023557 1_4h_MOI_1_untreated
GSM4023558 1_8h_MOI_1_untreated
GSM4023559 1_12h_MOI_1_untreated
BioProject PRJNA559467
SRA SRP217907

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Supplementary file Size Download File type/resource
GSE135619_RAW.tar 31.6 Mb (http)(custom) TAR (of CSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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