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Series GSE135481 Query DataSets for GSE135481
Status Public on Mar 23, 2021
Title Global landscape mapping of chromatin accessibility in DNA damage-induced senescent human stromal cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We report the application of an improved assay for transposase-accessible chromatin with high-throughput sequencing in profiling changes of chromatin openning state in senescent human stromal cells. By obtaining over four billion bases of sequence from Tn5 transposase-processed chromosome DNAs, we generated genome-wide chromatin-state maps of human primary stromal cells (with a starting cell number of 50,000). This study provides a framework for the application of ATAC-Seq technique towards spatiotemporal chromatin configuration of human stromal cells upon DNA damage-induced senescence.
Overall design Examination of 3 different cellular states in a primary normal human prosate stromal cell strain.
Contributor(s) Sun Y, Zhang B
Citation(s) 34263179
Boyi Zhang, Qilai Long, Shanshan Wu, Qixia Xu, Shuling Song, Liu Han, Min Qian, Xiaohui Ren, Hanxin Liu, Jing Jiang, Jianming Guo, Xiaoling Zhang, Xing Chang, Qiang Fu, Eric W-F Lam, Judith Campisi, James L Kirkland and Yu Sun. KDM4 orchestrates epigenomic remodeling of senescent cells and potentiates the senescence-associated secretory phenotype. Nat Aging 1, 454-472 (2021). doi:10.1038/s43587-021-00063-1
Submission date Aug 07, 2019
Last update date Jul 20, 2021
Contact name Yu Sun
Phone 86-21-54923302
Organization name Chinese Academy of Sciences
Department Shanghai Institute of Nutrition and Health
Lab Cancer Resistance
Street address 320 Yueyang Rd, Life Sciences Building A #1508
City Shanghai
ZIP/Postal code 200031
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (9)
GSM4011090 1--1: CTRL 01
GSM4011091 1--2: CTRL 02
GSM4011092 1--3: CTRL 03
BioProject PRJNA559076
SRA SRP217659

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Supplementary file Size Download File type/resource
GSE135481_RAW.tar 1.4 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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