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| Status |
Public on Mar 23, 2021 |
| Title |
Global landscape mapping of chromatin accessibility in DNA damage-induced senescent human stromal cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report the application of an improved assay for transposase-accessible chromatin with high-throughput sequencing in profiling changes of chromatin openning state in senescent human stromal cells. By obtaining over four billion bases of sequence from Tn5 transposase-processed chromosome DNAs, we generated genome-wide chromatin-state maps of human primary stromal cells (with a starting cell number of 50,000). This study provides a framework for the application of ATAC-Seq technique towards spatiotemporal chromatin configuration of human stromal cells upon DNA damage-induced senescence.
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| Overall design |
Examination of 3 different cellular states in a primary normal human prosate stromal cell strain.
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| Contributor(s) |
Sun Y, Zhang B |
| Citation(s) |
34263179 |
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Boyi Zhang, Qilai Long, Shanshan Wu, Qixia Xu, Shuling Song, Liu Han, Min Qian, Xiaohui Ren, Hanxin Liu, Jing Jiang, Jianming Guo, Xiaoling Zhang, Xing Chang, Qiang Fu, Eric W-F Lam, Judith Campisi, James L Kirkland and Yu Sun. KDM4 orchestrates epigenomic remodeling of senescent cells and potentiates the senescence-associated secretory phenotype. Nat Aging 1, 454-472 (2021). doi:10.1038/s43587-021-00063-1
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| Submission date |
Aug 07, 2019 |
| Last update date |
Jul 20, 2021 |
| Contact name |
Yu Sun |
| E-mail(s) |
sunyu@sinh.ac.cn, submarinesuny@yahoo.com
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| Phone |
86-21-54923302
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| Organization name |
Chinese Academy of Sciences
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| Department |
Shanghai Institute of Nutrition and Health
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| Lab |
Cancer Resistance
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| Street address |
320 Yueyang Rd, Life Sciences Building A #1508
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| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA559076 |
| SRA |
SRP217659 |
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